|
| Status |
Public on Aug 09, 2012 |
| Title |
MMTV-Cre:PTEN primary tumor CP243 |
| Sample type |
RNA |
| |
|
| Source name |
MMTV-Cre:PTEN primary tumor
|
| Organism |
Mus musculus |
| Characteristics |
strain background: mixed
genotype/variation: MMTV-Cre:PTEN
tissue: primary mammary tumor less than 1.0 cm diameter
|
| Treatment protocol |
Total RNA obtained from primary tumors were used for microarray analysis to determine differentially regulated genes among different mouse models. Cluster analysis and pathway analysis based on gene expression were then performed to determine significant genes/pathways
|
| Growth protocol |
Samples from independent primary mammary tumors from MMTV-Neu,MMTV-Cre:p53, MMTV-Cre:PTEN, MMTV-Cre:PTEN:p53, WAP-Cre:PTEN, and WAP-Cre:PTEN:p53 models were frozen and kept in -80 degree.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with Trizol reagent twice. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Affymetrix Mouse Gene 1.0 st microarray experiment were performed by the Centre of Applied Genomics (HSC, Toronto)
|
| |
|
| Hybridization protocol |
Affymetrix Mouse Gene 1.0 st microarray experiment were performed by the Centre of Applied Genomics (HSC, Toronto)
|
| Scan protocol |
Affymetrix Mouse Gene 1.0 st microarray experiment were performed by the Centre of Applied Genomics (HSC, Toronto)
|
| Description |
CP243
Gene expression data from primary MMTV-Cre:PTEN tumor
|
| Data processing |
Microarray Data were normalized using RMA method via Partek Genomics Suite (v.5.6) and absolute expression values were obtained
|
| |
|
| Submission date |
Aug 08, 2012 |
| Last update date |
Aug 09, 2012 |
| Contact name |
Jeff Liu |
| E-mail(s) |
jeff.liu@utoronto.ca
|
| Phone |
416-340-4800
|
| Organization name |
University Health Network
|
| Department |
Princess Margaret Cancer Center
|
| Lab |
Zadeh Lab
|
| Street address |
101 College Street, RM 4-601
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 1L7 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE39955 |
Expression data comparing Pten-, p53-, and combined deficient mouse mammary tumors |
|
