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Sample GSM98302 Query DataSets for GSM98302
Status Public on Dec 30, 2007
Title A/J_Skin_1h
Sample type RNA
 
Channel 1
Source name control RNA mixture
Organism Mus musculus
Characteristics Ten of First Choice total RNA (Ambion, Inc.) derived from ten organs(midterm embryo, thymus, ovary, testicle, spleen, lung, heart, liver, kidney, brain) were equally mixed.
Biomaterial provider Ambion, Inc.
Extracted molecule total RNA
Label Cy3
Label protocol Agilent Fluorescent Direct Label Kit Protocol (June 2003 Rev 2.1), Publication Number: G2555-98003_JUNE_2003
 
Channel 2
Source name Skin_1h
Organism Mus musculus
Characteristics 'Strain: A/J', 'Gender: female', 'Age: 12~13 week'
Treatment protocol One hour after local irrradiation (hind leg, 30Gy)
Extracted molecule total RNA
Extraction protocol ISOGEN(Wako pure chemical)+RNeasy(QIAGEN)
Label Cy5
Label protocol Agilent Fluorescent Direct Label Kit Protocol (June 2003 Rev 2.1), Publication Number: G2555-98003_JUNE_2003
 
 
Hybridization protocol cDNA Microarray Kit Protocol (Double barcoded slide) Version 7.0, Publication Number: G4100-98000
Scan protocol Agilent Microarray Scanner User Manual (6.3, 5th edition, December 2003), Publication Number: G2566-90009 10µm resolution, 100% PMT
Description In channel 2, total RNA was purified and pooled from 3 mice. Skin was taken as full layers.
Data processing Feature Extraction Software (Agilent Technologies)
 
Submission date Feb 24, 2006
Last update date Apr 22, 2007
Contact name Shuhei Noda
E-mail(s) s_noda@nirs.go.jp
Organization name National institute of radiological sciences
Department Frontier research center
Street address Anagawa 4-9-1
City Chiba
State/province Chiba
ZIP/Postal code 2658333
Country Japan
 
Platform ID GPL872
Series (1)
GSE4312 Radiation-induced genes commonly expressed in lung and skin among three strains of mice.

Data table header descriptions
ID_REF
VALUE LogRatio. log10(Dye normalized red(Cy5) Signal/Dye normalized green(Cy3) Signal) per feature
gMeanSignal Raw mean signal of feature in green(Cy3) channel
rMeanSignal Raw mean signal of feature in red(Cy5) channel
gBGMeanSignal Mean local background signal (local to corresponding feature) in green(Cy3) channel
rBGMeanSignal Mean local background signal (local to corresponding feature) in red(Cy5) channel

Data table
ID_REF VALUE gMeanSignal rMeanSignal gBGMeanSignal rBGMeanSignal
1 0.0455435 50273.8 65501.8 536.282 339.336
2 -0.259959 636.217 370.435 541.767 324.566
3 -0.0517621 626.556 415.296 539.474 322.839
4 -0.142228 636.04 396.36 540.719 322.055
5 -0.377302 573.273 330.773 540.742 323.359
6 -0.203983 580.077 355.462 537.889 319.963
7 -0.436787 577.222 326.222 538.878 323.183
8 -0.387652 795.885 400.615 541.818 322.045
9 -0.292617 613.88 355.72 542.568 319.356
12 0.265089 760.5 745.917 541.103 321.404
14 0.35392 931.88 1165.8 543.431 320.547
15 0.349881 769.643 861.25 543.674 318.326
16 -0.00664268 2090.78 1684.93 542.492 319.258
17 0.103288 1007.72 860.84 539.948 318.815
19 -0.148655 1723.05 1054.73 548.493 322.187
20 0.48768 913.96 1437.16 552.315 320.2
21 -0.422878 1884.6 751 552.47 319.343
22 -0.148481 699.615 429.769 556.023 319.459
23 -1.40148 2187 346.75 553.207 319.089
24 -0.114909 6444.13 4729.26 548.053 317.788

Total number of rows: 9275

Table truncated, full table size 413 Kbytes.




Supplementary data files not provided

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