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Sample GSM98303 Query DataSets for GSM98303
Status Public on Dec 30, 2007
Title A/J_Skin_12h
Sample type RNA
 
Channel 1
Source name control RNA mixture
Organism Mus musculus
Characteristics Ten of First Choice total RNA (Ambion, Inc.) derived from ten organs(midterm embryo, thymus, ovary, testicle, spleen, lung, heart, liver, kidney, brain) were equally mixed.
Biomaterial provider Ambion, Inc.
Extracted molecule total RNA
Label Cy3
Label protocol Agilent Fluorescent Direct Label Kit Protocol (June 2003 Rev 2.1), Publication Number: G2555-98003_JUNE_2003
 
Channel 2
Source name Skin_12h
Organism Mus musculus
Characteristics 'Strain: A/J', 'Gender: female', 'Age: 12~13 week'
Treatment protocol Twelve hours after local irrradiation (hind leg, 30Gy)
Extracted molecule total RNA
Extraction protocol ISOGEN(Wako pure chemical)+RNeasy(QIAGEN)
Label Cy5
Label protocol Agilent Fluorescent Direct Label Kit Protocol (June 2003 Rev 2.1), Publication Number: G2555-98003_JUNE_2003
 
 
Hybridization protocol cDNA Microarray Kit Protocol (Double barcoded slide) Version 7.0, Publication Number: G4100-98000
Scan protocol Agilent Microarray Scanner User Manual (6.3, 5th edition, December 2003), Publication Number: G2566-90009 10µm resolution, 100% PMT
Description In channel 2, total RNA was purified and pooled from 3 mice. Skin was taken as full layers.
Data processing Feature Extraction Software (Agilent Technologies)
 
Submission date Feb 24, 2006
Last update date Apr 22, 2007
Contact name Shuhei Noda
E-mail(s) s_noda@nirs.go.jp
Organization name National institute of radiological sciences
Department Frontier research center
Street address Anagawa 4-9-1
City Chiba
State/province Chiba
ZIP/Postal code 2658333
Country Japan
 
Platform ID GPL872
Series (1)
GSE4312 Radiation-induced genes commonly expressed in lung and skin among three strains of mice.

Data table header descriptions
ID_REF
VALUE LogRatio. log10(Dye normalized red(Cy5) Signal/Dye normalized green(Cy3) Signal) per feature
gMeanSignal Raw mean signal of feature in green(Cy3) channel
rMeanSignal Raw mean signal of feature in red(Cy5) channel
gBGMeanSignal Mean local background signal (local to corresponding feature) in green(Cy3) channel
rBGMeanSignal Mean local background signal (local to corresponding feature) in red(Cy5) channel

Data table
ID_REF VALUE gMeanSignal rMeanSignal gBGMeanSignal rBGMeanSignal
1 -0.352717 41904.2 61290.7 605.299 342.493
2 -0.345704 697.538 415.962 600.863 332.54
3 -0.375118 680.88 397.72 599.358 331.784
4 -0.191317 687.84 463.48 601.333 331.636
5 -0.473144 623.409 349.227 590.915 331.961
6 -0.297352 653.731 401.5 591.437 331.119
7 -0.503453 614.455 341.273 582.866 332.079
8 -0.391274 817.692 473.269 573.897 330.881
9 -0.342441 626.417 373.708 568.733 328.817
12 0.613469 745 1755.22 562.391 328.065
14 0.342751 982.792 1874.58 551.223 327.608
15 0.345261 765.4 1142.72 550.847 325.891
16 0.0259863 2128.81 3321.11 561.89 327.378
17 0.162709 1096.21 1607.17 561.345 326.791
19 -0.270845 1760.17 1422.17 559.168 327.092
20 0.640367 926.609 3047.43 563.679 327.382
21 -0.254372 1981.12 1709.77 565.418 325.522
22 -0.00842594 689.48 559.4 557.504 324.872
23 -1.55122 2226.28 370.56 555.992 323.039
24 -0.172589 6319 8982.8 574.772 327.596

Total number of rows: 9275

Table truncated, full table size 412 Kbytes.




Supplementary data files not provided

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