|
| Status |
Public on Aug 14, 2012 |
| Title |
Contents of midgut beetle 2 |
| Sample type |
RNA |
| |
|
| Source name |
midgut section
|
| Organism |
beetle metagenome |
| Characteristics |
sample type: content of midgut
|
| Treatment protocol |
Each beetle was surface washed with 95% EtOH and then washed with sterile PBS buffer. The beetles were dissected and each gut section was separated from the others, and placed in RNA later.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Each gut section was macerated and extracted using standard phenol-chloroform phase extraction, then followed by a cleanup with the AllPrep DNA/RNA kit (Qaigen).
|
| Label |
biotin
|
| Label protocol |
Method for biotin label were according to Brodie et al. (2006)
|
| |
|
| Hybridization protocol |
Method for hybridization were according to Brodie et al. (2006)
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Data processing |
The data were analyzed with GeneChip Microarray Analysis Suite version 5.1
|
| |
|
| Submission date |
Aug 13, 2012 |
| Last update date |
Aug 14, 2012 |
| Contact name |
Nhu Nguyen |
| E-mail(s) |
xerantheum@berkeley.edu
|
| Organization name |
University of California, Berkeley
|
| Department |
Plant and Microbial Biology
|
| Street address |
111 Koshland Hall
|
| City |
Berkeley |
| State/province |
CA |
| ZIP/Postal code |
94706 |
| Country |
USA |
| |
|
| Platform ID |
GPL15537 |
| Series (1) |
| GSE40067 |
Microbial communities in the gut of the beetle Odontotaenius disjunctus |
|
