|
| Status |
Public on Aug 14, 2012 |
| Title |
WT 5 Min heatshock |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
WT RNA 5 min 37C
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
genotype: wild type
time: 5min
treatment: 37C
genetic background: W303
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted via acid-phenol extraction
|
| Label |
Cy3
|
| Label protocol |
RNA was labeled with Agilent QuickAmp labeling kit.
|
| |
|
| Channel 2 |
| Source name |
WT 25 C
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
time: reference
genotype: wild type
treatment: 25C
genetic background: W303
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted via acid-phenol extraction
|
| Label |
Cy5
|
| Label protocol |
RNA was labeled with Agilent QuickAmp labeling kit.
|
| |
|
| |
| Hybridization protocol |
Equal masses of both sample and reference RNA were hybridized for 16-20 hours
|
| Scan protocol |
Scanned on a Agilent Technologies Scanner model G2505B
|
| Data processing |
Extraction Software: Agilent Feature Extraction, version 10.5.1.1. Opened on puma.db
|
| |
|
| Submission date |
Aug 13, 2012 |
| Last update date |
Aug 17, 2012 |
| Contact name |
Ana B Oromendia |
| E-mail(s) |
anao@mit.edu
|
| Organization name |
MIT- Koch Institute
|
| Lab |
Amon Lab
|
| Street address |
500 Main St
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platform ID |
GPL11488 |
| Series (1) |
| GSE40073 |
Disomic yeast response to heat shock |
|
