|
| Status |
Public on Aug 15, 2012 |
| Title |
spleen from Herts/33 group, rep2 |
| Sample type |
RNA |
| |
|
| Source name |
spleen from Herts/33-infected chicken, day 2 post infection
|
| Organism |
Gallus gallus |
| Characteristics |
breed: White Leghorn chickens
tissue: spleen
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 12 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
|
| Description |
Gene expression data of spleen from Herts/33-infected chicken
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100. Log2 transformation was applied to the MAS5 data.
|
| |
|
| Submission date |
Aug 14, 2012 |
| Last update date |
Aug 15, 2012 |
| Contact name |
Zenglei Hu |
| E-mail(s) |
zengleihu@163.com
|
| Phone |
+86-13773545421
|
| Organization name |
Yangzhou University
|
| Street address |
12 East Wenhui Road
|
| City |
yangzhou |
| ZIP/Postal code |
225009 |
| Country |
China |
| |
|
| Platform ID |
GPL3213 |
| Series (1) |
| GSE40100 |
Expression data of spleens from chickens infected with different Newcastle Disease Viruses |
|
