|
| Status |
Public on Nov 03, 2006 |
| Title |
Regulation of uptake hydrogenase and effects of hydrogen utilization on gene expression in Rhodopseudomonas palustris3 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Calibration experiment (#2-1)
|
| Organism |
Rhodopseudomonas palustris |
| Characteristics |
Mixed RNA from wild-type and hupV mutant grown under nitrogen-fixing conditions
|
| Extracted molecule |
total RNA |
| Label |
Cy3
|
| |
|
| Channel 2 |
| Source name |
Calibration experiment (#2-1)
|
| Organism |
Rhodopseudomonas palustris |
| Characteristics |
Mixed RNA from wild-type and hupV mutant grown under nitrogen-fixing conditions
|
| Extracted molecule |
total RNA |
| Label |
Cy5
|
| |
|
| |
| Description |
The software package lcDNA was used for data normalization and assessment of the statistical confidence intervals of gene expression. Duplicate calibration experiments and three comparative experiments using RNA from three separately grown cultures (three biological replicates) with duplicate slides for each (10 slides in total) were used to generate each data set.
|
| Data processing |
Images (Cy3 and Cy5) were captured as TIFF files and were analyzed with the image processing software ImaGene version 5.6 (BioDiscovery, Inc., El Segundo, CA).
|
| |
|
| Submission date |
Mar 01, 2006 |
| Last update date |
Nov 03, 2006 |
| Contact name |
Yasuhiro Oda |
| E-mail(s) |
yasuhiro@uw.edu
|
| Organization name |
University of Washington
|
| Department |
Department of Microbiology
|
| Lab |
Harwood
|
| Street address |
Box 357242, 1705 N.E. Pacific Street, HSB K-354
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98195-7242 |
| Country |
USA |
| |
|
| Platform ID |
GPL2697 |
| Series (1) |
| GSE4320 |
Regulation of uptake hydrogenase and effects of hydrogen utilization on gene expression in Rhodopseudomonas palustris |
|
