|
| Status |
Public on Sep 20, 2012 |
| Title |
DC_IFNα21_3days_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
IFNα21-DC at 3 days of differentiation
|
| Organism |
Homo sapiens |
| Characteristics |
ifn type1: IFNα21-DC
Stage: 3 days of differentiation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA were extracted with the High Pure RNA isolation Kit from Roche and according to manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the Affymetrix IVT Express protocol from 200 ng total RNA (3'IVT ExpressTechnical Manual, 2009, P/N702646 Rev1 Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 12 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 with HWS kit.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Data processing |
The values from the data matrix are MAS5 values and derived from affymetrix expression console software.
|
| |
|
| Submission date |
Aug 21, 2012 |
| Last update date |
Sep 20, 2012 |
| Contact name |
Geneviève Garcin |
| E-mail(s) |
genevieve.garcin@univ-montp2.fr
|
| Organization name |
CNRS UMR5235
|
| Lab |
DIMNP
|
| Street address |
UM2. Place Eugene Bataillon. Bat 24.
|
| City |
Montpellier |
| ZIP/Postal code |
34095 |
| Country |
France |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE40268 |
Expression data from 5 IFN type I-derived Dendritic Cells |
|
