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Status |
Public on Aug 23, 2013 |
Title |
whole bone marrow cells from gastric cancer patient (GC3) |
Sample type |
RNA |
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Source name |
whole bone marrow cells
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Organism |
Homo sapiens |
Characteristics |
gastric cancer patient: GC3 bone marrow fraction: whole age: 64 gender: Male tnm stage: IV distant metastasis: peritonitis carcinomatosa
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using miRNeasy Mini Kirt (QIAGEN) following manufacturer's instructions
|
Label |
Hy3
|
Label protocol |
Total RNA was labeled with miRCURY LNA microRNA Power Labeling kit (Exiqon) according to manufacture's protocol.
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|
|
Hybridization protocol |
Labeled RNA was hybridized to the microarray slide for 16-20 hr at 56 degrees C and washed with miRCURY LNA microRNA Power Labeling kit (Exiqon) according to manufacture's protocol.
|
Scan protocol |
Hybridization signals were detected by DNA microarray scanner G2505C (Agilent Technologies) with resolution of 10 micrometres . The scanned images were analyzed using Agilent Feature Extraction ver. 10.7.3.1.
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Data processing |
Each signal value of unique probe ID was calculated from average of Hy3 signal intensities of replicated probes.
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Submission date |
Aug 23, 2012 |
Last update date |
Aug 23, 2013 |
Contact name |
Ken Okano |
E-mail(s) |
rnai@cosmobio.co.jp
|
Organization name |
Cosmo Bio Co., LTD.
|
Street address |
Toyo Ekimae bldg. 2-20, Toyo 2-Chome, Koto-Ku
|
City |
Tokyo |
ZIP/Postal code |
135-0016 |
Country |
Japan |
|
|
Platform ID |
GPL11434 |
Series (1) |
GSE40325 |
microRNA expression profiles in bone marrow fractions from gastric cancer patients |
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