|
| Status |
Public on Aug 23, 2013 |
| Title |
CD45 positive bone marrow cells from gastric cancer patient (GC1) |
| Sample type |
RNA |
| |
|
| Source name |
CD45 positive bone marrow cells
|
| Organism |
Homo sapiens |
| Characteristics |
gastric cancer patient: GC1
bone marrow fraction: CD45 positive
age: 78
gender: Male
tnm stage: IV
distant metastasis: peritonitis carcinomatosa
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using miRNeasy Mini Kirt (QIAGEN) following manufacturer's instructions
|
| Label |
Hy3
|
| Label protocol |
Total RNA was labeled with miRCURY LNA microRNA Power Labeling kit (Exiqon) according to manufacture's protocol.
|
| |
|
| Hybridization protocol |
Labeled RNA was hybridized to the microarray slide for 16-20 hr at 56 degrees C and washed with miRCURY LNA microRNA Power Labeling kit (Exiqon) according to manufacture's protocol.
|
| Scan protocol |
Hybridization signals were detected by DNA microarray scanner G2505C (Agilent Technologies) with resolution of 10 micrometres .
The scanned images were analyzed using Agilent Feature Extraction ver. 10.7.3.1.
|
| Data processing |
Each signal value of unique probe ID was calculated from average of Hy3 signal intensities of replicated probes.
|
| |
|
| Submission date |
Aug 23, 2012 |
| Last update date |
Aug 23, 2013 |
| Contact name |
Ken Okano |
| E-mail(s) |
rnai@cosmobio.co.jp
|
| Organization name |
Cosmo Bio Co., LTD.
|
| Street address |
Toyo Ekimae bldg. 2-20, Toyo 2-Chome, Koto-Ku
|
| City |
Tokyo |
| ZIP/Postal code |
135-0016 |
| Country |
Japan |
| |
|
| Platform ID |
GPL11434 |
| Series (1) |
| GSE40325 |
microRNA expression profiles in bone marrow fractions from gastric cancer patients |
|
