|
| Status |
Public on Dec 31, 2012 |
| Title |
Bcells_normal_p53(246/-)_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
B cells_normal_p53(246/-)
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL6/129 hybrid
genotype of the cells: p53(246/-)
tissue origin: spleen
cell type: B cells
b cell type: normal
|
| Extracted molecule |
total RNA |
| Extraction protocol |
B cells were isolated by magnetic immuno-depletion method (MACS) and genotyped. Total RNA was extracted from all samples using Trizol and further purified by RNeasy RNA extraction kit (Qiagen). cDNA synthesis and amplification was performed using the WT cDNA synthesis and amplification kit (Nalgene).
|
| Label |
biotin
|
| Label protocol |
Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Nalgene)
|
| |
|
| Hybridization protocol |
Samples were hybridized using Affymetrix hybridization kit materials as per manufactorere's instructions
|
| Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G
|
| Description |
193P 2581.CEL
|
| Data processing |
Data were processed using Partek software (v.6.5). Quantile normalization uisng GC-RMS method, gene level expression were summarized from the quantile normalized data.
probe group file: MoEx-1_0-st-v1.r2.pgf
meta-probeset file: MoEx-1_0-st-v1.r2.dt1.mm8.mps
|
| |
|
| Submission date |
Aug 28, 2012 |
| Last update date |
Dec 31, 2012 |
| Contact name |
Kanaga Sabapathy |
| Organization name |
National Cancer Centre Singapore
|
| Street address |
11 Hospital Drive
|
| City |
Singapore |
| State/province |
Singapore |
| ZIP/Postal code |
169610 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL6096 |
| Series (2) |
| GSE40415 |
Acute and long-term effects of mutant p53 in vivo [B cells] |
| GSE40417 |
Acute and long-term effects of mutant p53 in vivo |
|
