tissue: brain, Left frontal Lobe histology: GBM tumor number (as appears in figure 3): 996 age: 45 gender: MALE grade: 4 living: DECEASED survival time: 224 survival cluster: SC2 hc_85_samples: HC2A array: 133B
Extracted molecule
total RNA
Extraction protocol
RNA extraction was performed as described previously (Mischel PS, Shai R, Shi T, et al Identification of molecular subtypes of glioblastoma by gene expression profiling. Oncogene, 2003;22:2361-73) and analyzed on both a spectrophotometer and Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA).
Label protocol
Probes were prepared using standard Affymetrix protocols and hybridized to Affymetrix HG-U133A and HG-U133B arrays (Affymetrix, Santa Clara, CA).
