|
Status |
Public on Jun 20, 2013 |
Title |
TFG-beta2, rep3 |
Sample type |
RNA |
|
|
Source name |
exosomes derived from cardiomyocytes, TGF-beta2 treated
|
Organism |
Mus musculus |
Characteristics |
cell type: exosomes derived from HL-1 cardiomyocytes treatment: TGF-beta2
|
Treatment protocol |
HL-1 cells were treated with or without growth factor (10ng/mL TGF-beta2 or 100ng/mL PDGF-BB, Invitrogen), and incubated in serum-free media for 23h before collection of media.
|
Growth protocol |
HL-1 cells were grown in T75 flasks and kept according to protocol (serum was ultracentrifuged before use).
|
Extracted molecule |
total RNA |
Extraction protocol |
Whole exosome RNA was purified with the RNeasy Fibrous Tissue Kit (Qiagen).
|
Label |
Cy3
|
Label protocol |
Illumina TotalPrep RNA Amplification Kit was used.
|
|
|
Hybridization protocol |
Performed according to Illumina protocol.
|
Scan protocol |
Illumina BeadStation 500GX according to Illumina protocol.
|
Description |
5773660151_D Exosomal RNA.
|
Data processing |
Cubic spline normalization, subtracted background. Illumina GenomeStudio was used.
|
|
|
Submission date |
Aug 30, 2012 |
Last update date |
Jun 20, 2013 |
Contact name |
Nina Gennebäck |
E-mail(s) |
nina.genneback@ncl.ac.uk
|
Organization name |
Umeå University
|
Department |
Public health and clinical medicin/medicin
|
Street address |
NUS 6M
|
City |
Umeå |
ZIP/Postal code |
901 86 |
Country |
Sweden |
|
|
Platform ID |
GPL6885 |
Series (1) |
GSE40503 |
Study of transcriptional content in exosomes derived from untreated and growth factor-treated cultured cardiomyocytes (HL-1) |
|