|
| Status |
Public on Dec 30, 2012 |
| Title |
CL1-5 1h 12/24 |
| Sample type |
RNA |
| |
|
| Source name |
IR 1h
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: CL1-5
tissue: lung adenocarcinoma
treatment: 10 Gy radiation
harvest time after exposure: 1h
|
| Treatment protocol |
irradiation
|
| Growth protocol |
Culture with RPMI medium
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from the cells were isolated using TRIzol® reagent (Invitrogen, Calsbad, CA) and purified with RNeasy® mini kits (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
5332143015_B
|
| Data processing |
Quantile normalization were carried out using Partek microarray analysis software.
|
| |
|
| Submission date |
Sep 04, 2012 |
| Last update date |
Dec 30, 2012 |
| Contact name |
Mong-Hsun Tsai |
| E-mail(s) |
motiont@gmail.com
|
| Phone |
+886-2-3366-6009
|
| Organization name |
National Taiwan University
|
| Department |
Institute of Biotechnology
|
| Street address |
4F, No. 81, Chang-Xing St.
|
| City |
Taipei |
| ZIP/Postal code |
106 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL8179 |
| Series (1) |
| GSE40602 |
MicroRNA-449a Enhances Radiosensitivity in Lung Adenocarcinoma Cells |
|
