|
| Status |
Public on Mar 21, 2013 |
| Title |
IL-2 treated (biol repl #1) |
| Sample type |
RNA |
| |
|
| Source name |
Kit225 cells treated with IL-2
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Leukemia cell
cell line: Kit225
|
| Treatment protocol |
Prior to IL-2 stimulation, Kit225 cells were made quiescent for 24 h in their regular medium without IL-2. Cell stimulations were carried out with 10 nM IL-2.
|
| Growth protocol |
Cells were maintained in RPMI-1640 medium containing 10% fetal calf serum, 2 mM L-glutamine and penicillin-streptomycin (50 IU/ml and 50 μg/ml, respectively). Kit225 media was supplemented with 20 U/ml human recombinant IL-2 (NCI Preclinical Repository).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed using Qiagen Rneasy Kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol in the Baylor College of Medicine Microarray Core Facility, Houston, TX.
|
| |
|
| Hybridization protocol |
Hybridization, etc was performed by the BCM Microarray Core Facility in Houston, TX.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data
|
| Data processing |
The raw data in the .CEL files were normalized by the GCRMA method (GC robust multi-array analysis). It is implemented in Bioconductor (http://www.bioconductor.org/). The raw signal intensity data were normalized, background corrected and summarized based on certain statistical models, and an expression value, in log2-scale, is obtained per chip per probe set.
|
| |
|
| Submission date |
Sep 05, 2012 |
| Last update date |
Mar 21, 2013 |
| Contact name |
Zsuzsanna S. Nagy |
| Organization name |
University of Debrecen
|
| Department |
Biochemistry and Molecular Biology
|
| Street address |
Nagyerdei krt. 98
|
| City |
Debrecen |
| ZIP/Postal code |
H-4032 |
| Country |
Hungary |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE40618 |
IL-2 induced gene expression changes in Kit225 human leukemia cell lines |
| GSE40624 |
Genome-wide mapping of IL-2 regulated target genes and IL-2 activated STAT5 binding sites in Kit225 human leukemia cells. |
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