DNA Probes
Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.
Year introduced: 1989
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Subheadings:
Tree Number(s): D13.444.600.223, D27.505.259.750.600.223, D27.720.470.530.600.223
MeSH Unique ID: D015342
Entry Terms:
- Probes, DNA
- DNA Probe
- Probe, DNA
- DNA Hybridization Probe
- Hybridization Probe, DNA
- Probe, DNA Hybridization
- DNA Hybridization Probes
- Hybridization Probes, DNA
- Probes, DNA Hybridization
- Chromosomal Probes
- Probes, Chromosomal
- Conserved Gene Probes
- Gene Probes, Conserved
- Probes, Conserved Gene
- Gene Probes, DNA
- DNA Gene Probes
- Probes, DNA Gene
- Whole Chromosomal Probes
- Chromosomal Probes, Whole
- Probes, Whole Chromosomal
- Whole Genomic DNA Probes
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