show Abstracthide AbstractRoot-knot nematodes of the genus Meloidogyne are obligatory plant endoparasites that cause substantial economic losses to the agricultural production and impact the global food supply. We report a high-quality genome assembly of Meloidogyne enterolobii using the high-fidelity long-read sequencing approach, developed by Pacific Biosciences, combined with a gap-aware sequence transformer, DeepConsensus. The resulting genome assembly spans 273 Mbp with 556 contigs, a GC% of 30 ± 0.042 and an N50 value of 2.11Mb, constituting a useful platform for comparative, population and functional genomics. Thus enabling us to perform high quality annotations of coding and non-coding genes.