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DRX307866: Illumina HiSeq 2500 sequencing of SAMD00404140
1 ILLUMINA (Illumina HiSeq 2500) run: 29.2M spots, 1.5G bases, 652.8Mb downloads

Submitted by: KYOTO-GB
Study: Transcriptome analysis and small RNA analysis of poly (I:C)-treated Eptesicus bat cells
show Abstracthide Abstract
This study aims to investigate the gene expression profiles of immune responses in Eptesicus bat cells, Efk3B from E. fuscus and EnK from E. nilsonni. We treated Efk3B and EnK with poly (I:C) to induce immune responses. The total RNA from Efk3B as well as EnK and the total small RNA from Efk3B were extracted for high-throughput sequencing. The differentially expressed genes (DEGs), transposable elements (TEs, DETs), and microRNAs (miRNAs) were further identified and analyzed. We identified the extraordinary mechanisms of immune responses genes in bat cells, including the uniquely up-regulated genes, induced but unannotated transcripts, and higher basal expression level of immunity-related genes. Moreover, we revealed the differentially expressed TEs (DETs) and novel miRNAs that might be related to the immune responses. In conclusion, this study comprehensively demonstrates the gene expression profiles and provides new insights into the immune responses in Eptesicus bat cell lines.
Sample: Small RNA sequencing of untreated Efk3B replicate 2
SAMD00404140 • DRS325695 • All experiments • All runs
Library:
Name: Efk3B_sRNA_untreated_2
Instrument: Illumina HiSeq 2500
Strategy: miRNA-Seq
Source: TRANSCRIPTOMIC
Selection: size fractionation
Layout: SINGLE
Construction protocol: To prepare the single-end small RNA libraries, total RNA including small RNAs was extracted using Direct-zol RNA Kits (Zymo Research). The libraries were constructed using TruSeq Small RNA Library Prep Kit (Illumina) with protocol "TruSeq Small RNA Library Prep Guide, Part # 15004197 Rev. G".
Spot descriptor:
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Runs: 1 run, 29.2M spots, 1.5G bases, 652.8Mb
Run# of Spots# of BasesSizePublished
DRR31851029,192,3341.5G652.8Mb2023-09-09

ID:
29381260

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