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SRX258241: Legionella pneumophila subsp. pneumophila str. Philadelphia 1 transformed with pPpacS-EGFP clone 4
1 ILLUMINA (Illumina HiSeq 2500) run: 1.3M spots, 262.6M bases, 183.7Mb downloads

Design: Genomic DNA was prepared from post-exponential (A600 nm > 4) bacteria using the Qiagen DNeasy kit, sheared to ~400 bp using a Covaris S2 ultrasonicator, blunted with the End-IT DNA Repair kit (Epicentre), and A-tailed using Exo-minus Klenow (Lucigen) and dATP. Custom adapter sequences with 8 nucleotide indices located 3' of each insert were ligated to the A-tailed fragments using the Fast-link ligation kit (Epicentre). Ligated DNA of approximately 500 - 600 nt of total length was isolated by 2% agarose TAE gel electrophoresis, with narrow bands excised using GeneCatcher disposable gel excision tips (the Gel Company) and purified using the Machery Nagel NucleoSpin kit. To enrich for properly ligated samples, approximately 4% of the library was amplified for 16 cycles using common primers.
Submitted by: University of Toronto
Study: Legionella pneumophila subsp. pneumophila str. Philadelphia 1 Genome sequencing
PRJNA195914 • SRP020472 • All experiments • All runs
show Abstracthide Abstract
Comparative genomics of the laboratory strains derived from Legionella pneumophila subsp. pneumophila str. Philadelphia 1.
Sample: General Sample for Legionella pneumophila subsp. pneumophila str. Philadelphia 1 sample Phila1_Clin_GFP4
SAMN01994702 • SRS406358 • All experiments • All runs
Organism: Legionella pneumophila subsp. pneumophila str. Philadelphia 1
Library:
Name: Phila1_Clin_GFP4
Instrument: Illumina HiSeq 2500
Strategy: WGS
Source: GENOMIC
Selection: RANDOM
Layout: PAIRED
Spot descriptor:
1  forward101  reverse

Runs: 1 run, 1.3M spots, 262.6M bases, 183.7Mb
Run# of Spots# of BasesSizePublished
SRR8018291,312,968262.6M183.7Mb2013-05-30

ID:
361210

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