GSM1835818: ChIPSeq_Input_E8.5 WT-Rep3; Mus musculus; ChIP-Seq - SRA

SRX1123798: GSM1835818: ChIPSeq_Input_E8.5 WT-Rep3; Mus musculus; ChIP-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 93.6M spots, 4.7G bases, 2.6Gb downloads

Submitted by: Gene Expression Omnibus (GEO)

Study: Study of the impact of G9a on DNA methylation in mouse embryonic cells [seq]

PRJNA291405 • SRP061767 • All experiments • All runs

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We explored the role of the lysine-methyltrasferase G9a in the control of DNA methylation during mouse embryogenesis. We provide maps of cytosine methylation by MeDIP and RRBS in G9a-deficient mouse embryos and ES cells, as well as ChIP-Seq profiles for H3K9me2 in embryos and RNA-Seq expression profiles in G9a-deficient embryos. Overall design: We prepared RRBS libraries from single embryos and mapped DNA methylation by RRBS in four WT and three G9aKO E8.5 embryos. We prepared RNA-Seq libraries from RNAs of single embryos and mapped the transcriptome of two WT and two G9aKO E8.5 embryos. We prepared H3K9me2 ChIP-Seq libraries from three independant ChIP experiments on eight pooled E8.5 embryos.

Sample: ChIPSeq_Input_E8.5 WT-Rep3

SAMN03943761 • SRS1016210 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: Illumina HiSeq 2500

Strategy: ChIP-Seq

Source: GENOMIC

Selection: ChIP

Layout: SINGLE

Construction protocol: 8 embryos collected at E8.5 were dissociated in Trypsin 0.25% EDTA 1mM for 5 min at RT, washed in PBS and cross-linked with 1 % formaldehyde for 10 min at RT. After sonication with a Bioruptor waterbath sonicator (Diagenode), we performed ChIP with the LowCell ChIP kit (Diagenode). ChIP-Seq libraries were prepared with the NEXTflex ChIP-Seq Kit (Bioo Scientific) according to the manufacturer’s instructions.

Experiment attributes:

GEO Accession: GSM1835818

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 93.6M spots, 4.7G bases, 2.6Gb

Run	# of Spots	# of Bases	Size	Published
SRR2133441	93,607,517	4.7G	2.6Gb	2015-11-13

ID:: 1639603

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