Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Brain tissue was flash frozen on dry ice. mRNA was extracted using the Trizol Plus RNA Purification Kit, with DNase treatment with the Ambion RNAse-Free DNase Set and column cleanup with the PureLink RNA Mini Kit (Invitrogen, Carlsbad, CA USA). cDNA libraries were synthesized using the TruSeq RNA Sample Preparationv2 kit (Illumina) with TruSeq Indexes.