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SRX2207634: GSM2333660: y53; Catharus ustulatus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 47.7M spots, 9.5G bases, 6.1Gb downloads

Submitted by: NCBI (GEO)
Study: Seasonal gene expression in a migratory songbird
show Abstracthide Abstract
We sequenced mRNA from brain samples of 20 captive Swainson''s thrushes. 10 were from California, belonging to the coastal subspecies, and 10 were from Alaska, belonging to the inland subspecies. Overall design: Gene expression level analysis of Swainson''s thrushes maintained in captivity for >1 year. The study treatments were: non-migratory inland (N = 4), non-migratory coastal (N = 6), migratory inland (N = 6), migratory coastal (N = 4). This Series includes 19 Samples. This Series does not include migratory inland Sample t22 (it was not included in any analyses because its overall expression was an outlier to the remaining dataset).
Sample: y53
SAMN05858772 • SRS1725203 • All experiments • All runs
Library:
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Brain tissue was flash frozen on dry ice. mRNA was extracted using the Trizol Plus RNA Purification Kit, with DNase treatment with the Ambion RNAse-Free DNase Set and column cleanup with the PureLink RNA Mini Kit (Invitrogen, Carlsbad, CA USA). cDNA libraries were synthesized using the TruSeq RNA Sample Preparationv2 kit (Illumina) with TruSeq Indexes.
Experiment attributes:
GEO Accession: GSM2333660
Links:
Runs: 1 run, 47.7M spots, 9.5G bases, 6.1Gb
Run# of Spots# of BasesSizePublished
SRR434033247,686,5779.5G6.1Gb2016-12-15

ID:
3243114

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