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SRX3181548: GSM2781592: HL-48 Xylose Rep3; Halomonas sp. HL48; RNA-Seq
6 ABI_SOLID (AB 5500xl Genetic Analyzer) runs: 22.6M spots, 1.1G bases, 650.3Mb downloads

Submitted by: NCBI (GEO)
Study: Phenotypic responses to interspecies competition and commensalism in a naturally-derived microbial co-culture
show Abstracthide Abstract
Testing of response of hetertrophic partners (Halomonas sp. HL-48 and Marinobacter sp. HL-58) to both axenic and co-culture growth using both glucose and xylose Overall design: Both Halomonas sp. HL-48 and Marinobacter sp. HL-58 were grown either on glucose axenically or in co-culture and on xylose in co-culture. Halomonas HL-48 was also grown on xylose axenically. Transcriptomic data was collected under each condition and used to determine each organisms response to the other under both competitive and commensal conditions.
Sample: HL-48 Xylose Rep3
SAMN07637234 • SRS2510279 • All experiments • All runs
Library:
Instrument: AB 5500xl Genetic Analyzer
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: RNA was extracted using Trizol following by DNAseI treatment using Qiagen RNAseay mini column Template cDNA was prepared using the Applied Biosystems SOLiD® Total RNA-Seq Kit (Life Technologies, Carlsbad, CA) according to manufacturer's protocol. Sequencing was carried using the SOLiDTM 5500XL protocol (Life Technologies).
Experiment attributes:
GEO Accession: GSM2781592
Links:
Runs: 6 runs, 22.6M spots, 1.1G bases, 650.3Mb
Run# of Spots# of BasesSizePublished
SRR60312873,814,708190.7M110.2Mb2017-09-14
SRR60312883,704,955185.2M108.2Mb2017-09-14
SRR60312893,745,693187.3M107.2Mb2017-09-14
SRR60312903,841,860192.1M109.5Mb2017-09-14
SRR60312913,683,448184.2M105.7Mb2017-09-14
SRR60312923,827,093191.4M109.5Mb2017-09-14

ID:
4483501

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