GSM2781586: HL-48 and HL-58 Glucose Rep2; Halomonas sp. HL48; Halomon... - SRA

SRX3181542: GSM2781586: HL-48 and HL-58 Glucose Rep2; Halomonas sp. HL48; Halomonas sp. HL58; RNA-Seq
6 ABI_SOLID (AB 5500xl Genetic Analyzer) runs: 83.6M spots, 4.2G bases, 2.4Gb downloads

Submitted by: NCBI (GEO)

Study: Phenotypic responses to interspecies competition and commensalism in a naturally-derived microbial co-culture

PRJNA406985 • SRP117464 • All experiments • All runs

show Abstracthide Abstract

Testing of response of hetertrophic partners (Halomonas sp. HL-48 and Marinobacter sp. HL-58) to both axenic and co-culture growth using both glucose and xylose Overall design: Both Halomonas sp. HL-48 and Marinobacter sp. HL-58 were grown either on glucose axenically or in co-culture and on xylose in co-culture. Halomonas HL-48 was also grown on xylose axenically. Transcriptomic data was collected under each condition and used to determine each organisms response to the other under both competitive and commensal conditions.

Sample: HL-48 and HL-58 Glucose Rep2

SAMN07637240 • SRS2510273 • All experiments • All runs

Organism: Halomonas sp. HL48

Library:

Instrument: AB 5500xl Genetic Analyzer

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: RNA was extracted using Trizol following by DNAseI treatment using Qiagen RNAseay mini column Template cDNA was prepared using the Applied Biosystems SOLiD® Total RNA-Seq Kit (Life Technologies, Carlsbad, CA) according to manufacturer's protocol. Sequencing was carried using the SOLiDTM 5500XL protocol (Life Technologies).

Experiment attributes:

GEO Accession: GSM2781586

Links:

NCBI link: NCBI Entrez (gds)

Runs: 6 runs, 83.6M spots, 4.2G bases, 2.4Gb

Run	# of Spots	# of Bases	Size	Published
SRR6031251	13,303,650	665.2M	386.3Mb	2017-09-14
SRR6031252	12,322,407	616.1M	376.1Mb	2017-09-14
SRR6031253	13,422,421	671.1M	390Mb	2017-09-14
SRR6031254	14,416,099	720.8M	423.4Mb	2017-09-14
SRR6031255	14,309,776	715.5M	422.5Mb	2017-09-14
SRR6031256	15,777,435	788.9M	457.1Mb	2017-09-14

ID:: 4483495

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