GSM2834610: sw-cbf123 24hr-cold rep1; Arabidopsis thaliana; RNA-Seq - SRA

SRX3339035: GSM2834610: sw-cbf123 24hr-cold rep1; Arabidopsis thaliana; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 23.6M spots, 1.2G bases, 550.2Mb downloads

Submitted by: NCBI (GEO)

Study: Potential role of the CBF Pathway contributing to local adaptation of ecotypes collected from Italy and Sweden

PRJNA416120 • SRP122518 • All experiments • All runs

show Abstracthide Abstract

The two Arabidopsis ecotypes that are adapted to Italy and Sweden, respectively, showed different degree of freezing tolerance. By comparing the low temperature transcriptomes of the IT and SW ecotypes with the CBF pathway being knocked out, we showed the CBF pathway plays a significant role in natural variation of freezing tolerance. Overall design: Examination of mRNA levels in cbf CRISPR mutants in background of the Arabidospsis Italian and Swedish ecotype plants grown at warm, cold for 24h, and cold for 2 weeks.

Sample: sw-cbf123 24hr-cold rep1

SAMN07842298 • SRS2641111 • All experiments • All runs

Organism: Arabidopsis thaliana

Library:

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: RNA was extracted using the RNeasy plant mini kit (Qiagen) RNA libraries were prepared for sequencing using standard Illumina protocols

Experiment attributes:

GEO Accession: GSM2834610

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 23.6M spots, 1.2G bases, 550.2Mb

Run	# of Spots	# of Bases	Size	Published
SRR6230521	23,605,352	1.2G	550.2Mb	2018-07-31

ID:: 4668164

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