GSM3760134: 53A_ILC3; Homo sapiens; RNA-Seq - SRA

SRX5818178: GSM3760134: 53A_ILC3; Homo sapiens; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 3.8M spots, 223.5M bases, 101Mb downloads

Submitted by: NCBI (GEO)

Study: Group 3 innate lymphoid cells mediate early protective immunity against Mycobacterium tuberculosis

PRJNA542348 • SRP197580 • All experiments • All runs

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We report the phenotype of human lung ILC2 and ILC3 populations from individuals with tuberculosis (TB) and non-TB cancer controls. We find that ILC2s demonstrate moderate transcriptional differences in TB infection, whereas ILC3s demonstrate large differences. Overall design: ILC2s and ILC3s were purified by FACS from lung biopsies from TB infected lung tissue and peripheral healthy lung tissue from individuals with cancer. Low-input RNA-seq was performed on 1-3 replicates (dependent on cell number) on 5 individuals with TB infection and 2 controls.

Sample: 53A_ILC3

SAMN11619012 • SRS4746947 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Total RNA was extracted from cell lysate (RLT +1% BME) using RNAClean XP SPRI beads. Messenger RNA was primed for reverse transcription and PCR using an oligodT RT primer. Libraries were constructed using a modified version of the Smart-Seq2 protocol described in Trombetta et al., Curr Protoc Mol Biol, (2014). Libraries were sequenced twice on a NextSeq 500 using the 75-cycle High Throughput kit.

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 3.8M spots, 223.5M bases, 101Mb

Run	# of Spots	# of Bases	Size	Published
SRR9041082	3,785,024	223.5M	101Mb	2019-05-13

ID:: 7819219

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