Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: RNA Extraction. Total RNA was extracted from those samples using the mirVana RNA Isolation Kit (Life Technologies, Grand Island, NY). Samples were bead-beaten for 1 min at maximum speed with 300 μl of 0.1-mm zirconia-silica beads (BioSpec Products, Bartlesville, Okla.) in the mirVana lysis buffer. Bacterial ribosomal RNA was depleted using Ribo-Zero rRNA Removal Kits (Bacteria) (Epicentre, Madison, WI) following the manufacturer's protocol. For RNA processing for eukaryotic analysis, we used Dynabeads mRNA Purification Kit to isolate eukaryotic mRNA for transcriptome analysis. RNA libraries were prepared for sequencing using standard Illumina protocols. For RNA sequencing, Illumina adapter-specific primers were used to amplify and selectively enrich for the cDNA generated from enriched mRNA. Quantified libraries were pooled and sequenced using a HiSeq 2500 machine, 2x100 Flow-cell (Illumina). The Nextera XT kit was used to generate libraries from amplified DNA.