show Abstracthide AbstractCurrently, there is no reference genome for Aphanomyces cochlioides. Our objective was to assemble and annotate a reference genome for A. cochlioides to publish as a resource to facilitate future research into the biology of this sugar beet pathogen. To accomplish this, we conducted a de novo genome assembly for A. cochlioides using Nanopore long reads, and corrected by Illumina short reads. The genome was annotated with RNA-seq reads from various tissues, infected sugar beet seedlings, and available Aphanomyces spp. RNA and protein sequences.