U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

SRX12834649: GSM5661564: KOD1-KO-Small-RNA-sample; Thermococcus kodakarensis KOD1; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 72.7M spots, 7.4G bases, 4.2Gb downloads

Submitted by: NCBI (GEO)
Study: Genetic and functional analysis of archaeal ATP-dependent RNA ligase in ribosomal RNA biogenesis
PRJNA776174 • SRP343732 • All experiments • All runs
show Abstracthide Abstract
In this study, we generated allelic knockouts of ATP-dependent RNA ligase (Rnl) in hyperthermophilic archaeon T. kodakarensis and analyze the total and small RNA by RNA-Seq. Overall design: Knocked out the TK1545 gene by homologous recombination, and prepared RNA samples for whole transcriptosome and small RNA seq analysis. The differentially expressed genes between the two strains were analyzed.
Sample: KOD1-KO-Small-RNA-sample
SAMN22771676 • SRS10779480 • All experiments • All runs
Organism: Thermococcus kodakarensis KOD1
Library:
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: T. kodakarensis total RNA was isolated from WT and tk1545KO cultures using TRIZOL reagent (Invitrogen) RNA libraries were prepared for sequencing using standard Illumina protocols
Experiment attributes:
GEO Accession: GSM5661564
Links:
Runs: 1 run, 72.7M spots, 7.4G bases, 4.2Gb
Run# of Spots# of BasesSizePublished
SRR1663362772,673,1387.4G4.2Gb2021-11-02

ID:
17493717

Supplemental Content

Related information

Search details

See more...

Recent activity

Clear Turn Off Turn On

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...