U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

SRX25513471: whole-genome sequencing of chikungunya virus vaccine strain to examine RdRP fidelity in nsP4 variants
1 ILLUMINA (NextSeq 2000) run: 480,000 spots, 40.1M bases, 13.8Mb downloads

Design: viral rna extraction followed by random-primed cdna synthesis and on-bead tagmentation with illumina S beads and library amplification with EPM. paired end reads quality trimmed and merged
Submitted by: Greninger (Alex)
Study: Deep sequencing of chikungunya virus vaccine strain 181/25 cultures with nsP4 variants
PRJNA1141983 • SRP523089 • All experiments • All runs
show Abstracthide Abstract
Data reported in Mutations in Chikungunya Virus nsP4 Decrease Sensitivityto the Broad-Spectrum Antiviral 4'-Fluorouridine. Chikungunya virus vaccine strain 181/25 with nsp4 C483 and nsp4 Y483 were cultured, RNA extracted, and deep sequenced to measure low-frequency mutations to compare fidelity of these RdRP variants.
Sample:
SAMN42914210 • SRS22167677 • All experiments • All runs
Organism: Chikungunya virus
Library:
Name: 231018_Einstein_PY_010_C636_WT_1
Instrument: NextSeq 2000
Strategy: WGS
Source: VIRAL RNA
Selection: cDNA_randomPriming
Layout: SINGLE
Runs: 1 run, 480,000 spots, 40.1M bases, 13.8Mb
Run# of Spots# of BasesSizePublished
SRR30037983480,00040.1M13.8Mb2024-07-30

ID:
34495103

Supplemental Content

Related information

Search details

See more...

Recent activity

Clear Turn Off Turn On

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...