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SRX2188250: Whole genome sequencing of C. lanienae strain RM10695
1 ILLUMINA (Illumina MiSeq) run: 396,111 spots, 98M bases, 51.8Mb downloads

Design: Illumina libraries were prepared for all strains using the KAPA Low-Throughput Library Preparation Kit with Standard PCR Amplification Module (Kapa Biosystems, Wilmington, MA), following manufacturer's instructions except for the following changes: 750 ng DNA was sheared at 30 psi for 40 s and size selected to 700-770 bp following Illumina protocols. Standard desalting TruSeq LT and PCR Primers were ordered from Integrated DNA Technologies (Coralville, IA) and used at 0.375 and 0.5 ľM final concentrations, respectively. PCR was reduced to 3-5 cycles. Libraries were quantified using the KAPA Library Quantification Kit (Kapa), except with 10 ľl volume and 90-s annealing/extension PCR, then pooled and normalized to 4 nM. Pooled libraries were requantified by ddPCR on a QX200 system (Bio-Rad), using the Illumina TruSeq ddPCR Library Quantification Kit and following manufacturer's protocols, except with an extended 2-min annealing/extension time. The libraries were sequenced 2 × 250 bp paired end v2 on a MiSeq instrument (Illumina) at 13.5 pM, following manufacturer's protocols.
Submitted by: USDA, ARS, WRRC
Study: Campylobacter lanienae strain:RM10695 Genome sequencing and assembly
PRJNA341007 • SRP090368 • All experiments • All runs
show Abstracthide Abstract
Genomic and taxonomic analysis of Campylobacter lanienae and C. lanienae related organisms
Sample:
SAMN05687027 • SRS1711041 • All experiments • All runs
Organism: Campylobacter lanienae
Library:
Name: RM10695WGS_MiSeq
Instrument: Illumina MiSeq
Strategy: WGS
Source: GENOMIC
Selection: RANDOM
Layout: SINGLE
Runs: 1 run, 396,111 spots, 98M bases, 51.8Mb
Run# of Spots# of BasesSizePublished
SRR4292773396,11198M51.8Mb2017-10-01

ID:
3183476

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