5O9Z: Cryo-EM structure of a pre-catalytic human spliceosome primed for activation (B complex)

PDB ID: 5O9ZDownload
MMDB ID: 153654
PDB Deposition Date: 2017/6/20
Updated in MMDB: 2022/11
Experimental Method:
electron microscopy
Resolution: 4.5  Å
Source Organism:
Similar Structures:
Molecular Components in 5O9Z
Label Count Molecule
Proteins (52 molecules)
1
Pre-mrna-processing-splicing Factor 8
(Gene symbol: PRPF8)
Molecule annotation
1
116 KDA U5 Small Nuclear Ribonucleoprotein Component
(Gene symbol: EFTUD2)
Molecule annotation
1
U5 Small Nuclear Ribonucleoprotein 200 KDA Helicase
(Gene symbol: SNRNP200)
Molecule annotation
1
U5 Small Nuclear Ribonucleoprotein 40 KDA Protein
(Gene symbol: SNRNP40)
Molecule annotation
1
U4/u6 Small Nuclear Ribonucleoprotein Prp3
(Gene symbol: PRPF3)
Molecule annotation
1
U4/u6 Small Nuclear Ribonucleoprotein Prp4
(Gene symbol: PRPF4)
Molecule annotation
1
Pre-mrna-processing Factor 6
(Gene symbol: PRPF6)
Molecule annotation
1
U4/u6 Small Nuclear Ribonucleoprotein Prp31
(Gene symbol: PRPF31)
Molecule annotation
1
Pre-mrna-splicing Factor 38A
(Gene symbol: PRPF38A)
Molecule annotation
1
Thioredoxin-like Protein 4A
(Gene symbol: TXNL4A)
Molecule annotation
1
Microfibrillar-associated Protein 1
(Gene symbol: MFAP1)
Molecule annotation
1
Wd40 Repeat-containing Protein Smu1
(Gene symbol: SMU1)
Molecule annotation
1
Peptidyl-prolyl Cis-trans Isomerase H
(Gene symbol: PPIH)
Molecule annotation
1
Zinc Finger Matrin-type Protein 2
(Gene symbol: ZMAT2)
Molecule annotation
1
Nhp2-like Protein 1
(Gene symbol: SNU13)
Molecule annotation
1
U4/u6.u5 Tri-snrnp-associated Protein 1
(Gene symbol: SART1)
Molecule annotation
1
WW Domain-binding Protein 4
(Gene symbol: WBP4)
Molecule annotation
1
Protein RED
(Gene symbol: IK)
Molecule annotation
3
Small Nuclear Ribonucleoprotein SM D2
(Gene symbol: SNRPD2)
Molecule annotation
3
Small Nuclear Ribonucleoprotein F
(Gene symbol: SNRPF)
Molecule annotation
3
Small Nuclear Ribonucleoprotein E
(Gene symbol: SNRPE)
Molecule annotation
3
Small Nuclear Ribonucleoprotein G
(Gene symbol: SNRPG)
Molecule annotation
3
Small Nuclear Ribonucleoprotein SM D3
(Gene symbol: SNRPD3)
Molecule annotation
3
Small Nuclear Ribonucleoprotein-associated Proteins B and B'
(Gene symbol: SNRPB)
Molecule annotation
3
Small Nuclear Ribonucleoprotein SM D1
(Gene symbol: SNRPD1)
Molecule annotation
1
U6 Snrna-associated Sm-like Protein Lsm2
(Gene symbol: LSM2)
Molecule annotation
1
U6 Snrna-associated Sm-like Protein Lsm3
(Gene symbol: LSM3)
Molecule annotation
1
U6 Snrna-associated Sm-like Protein Lsm4
(Gene symbol: LSM4)
Molecule annotation
1
U6 Snrna-associated Sm-like Protein Lsm5
(Gene symbol: LSM5)
Molecule annotation
1
U6 Snrna-associated Sm-like Protein Lsm6
(Gene symbol: LSM6)
Molecule annotation
1
U6 Snrna-associated Sm-like Protein Lsm7
(Gene symbol: LSM7)
Molecule annotation
1
U6 Snrna-associated Sm-like Protein Lsm8
(Gene symbol: LSM8)
Molecule annotation
1
Splicing Factor 3B Subunit 1
(Gene symbol: SF3B1)
Molecule annotation
1
Splicing Factor 3B Subunit 3 (Sf3b3)
(Gene symbol: SF3B3)
Molecule annotation
1
Splicing Factor 3B Subunit 5
(Gene symbol: SF3B5)
Molecule annotation
1
PHD Finger-like Domain-containing Protein 5A
(Gene symbol: PHF5A)
Molecule annotation
1
U2 Small Nuclear Ribonucleoprotein A'
(Gene symbol: SNRPA1)
Molecule annotation
1
U2 Small Nuclear Ribonucleoprotein B''
(Gene symbol: SNRPB2)
Molecule annotation
Nucleotides(5 molecules)
1
Minx Pre-mrna
Molecule annotation
1
Human Gene for Small Nuclear RNA U2 (Snrna U2)
Molecule annotation
1
Homo Sapiens U4A Snrna
Molecule annotation
1
Homo Sapiens U5 a Small Nuclear RNA
Molecule annotation
1
Homo Sapiens RNA, U6 Small Nuclear 1 (Rnu6-1), Small Nuclear RNA
Molecule annotation
* Click molecule labels to explore molecular sequence information.
Citing MMDB
Madej T, Lanczycki CJ, Zhang D, Thiessen PA, Geer RC, Marchler-Bauer A, Bryant SH. " MMDB and VAST+: tracking structural similarities between macromolecular complexes. Nucleic Acids Res. 2014 Jan; 42(Database issue):D297-303