9JKQ: Cryo-EM structure of the METH-bound hTAAR1-Gs complex

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PDB ID: 9JKQDownload
MMDB ID: 253808
PDB Deposition Date: 2024/9/16
Updated in MMDB: 2024/10
Experimental Method:
electron microscopy
Resolution: 2.66  Å
Source Organism:
synthetic construct
Similar Structures:
Biological Unit for 9JKQ: pentameric; determined by author and by software
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9JKQ: Cryo-EM structure of the METH-bound hTAAR1-Gs complex
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AA: Guanine nucleotide-binding protein G(s) subunit alpha isoforms shortBB: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1CC: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2NN: Nb35RR: Soluble cytochrome b562,Trace amine-associated receptor 11B40: (2S)-N-methyl-1-phenylpropan-2-amine
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Molecular Components in 9JKQ
Label Count Molecule
Proteins (5 molecules)
1
Guanine Nucleotide-binding Protein G(s) Subunit Alpha Isoforms Short
1 Protein
SS Diagram
3D Domains
Features
Domain Families
Specific Hits
Super Families
1100200300394394 residues, click to see VAST similar structures.helix 10-39helix 265-278helix 294-302helix 332-349helix 370-391strand 40-47strand 206-215strand 216-225strand 242-250strand 284-293strand 356-365Domain 1 (40-394): click to see VAST similar structures1GTP/Mg2+ binding siteGTP/Mg2+ binding(12)location: 47, residue: Glocation: 52, residue: Glocation: 53, residue: Klocation: 54, residue: Nlocation: 204, residue: Tlocation: 223, residue: Dlocation: 226, residue: Alocation: 292, residue: Nlocation: 293, residue: Klocation: 295, residue: Dlocation: 365, residue: Clocation: 366, residue: Aadenylyl cyclase interaction siteadenylyl cyclase interaction site(5)location: 207, residue: Ilocation: 232, residue: Rlocation: 235, residue: Ilocation: 236, residue: Qlocation: 239, residue: Nbeta - gamma complex interaction sitebeta - gamma complex interaction site(15)location: 205, residue: Slocation: 207, residue: Ilocation: 209, residue: Elocation: 220, residue: Hlocation: 222, residue: Flocation: 226, residue: Alocation: 227, residue: Qlocation: 228, residue: Rlocation: 230, residue: Elocation: 233, residue: Klocation: 234, residue: Wlocation: 236, residue: Qlocation: 237, residue: Clocation: 238, residue: Flocation: 239, residue: NGoLoco binding siteGoLoco binding site(16)location: 48, residue: Alocation: 49, residue: Glocation: 50, residue: Elocation: 93, residue: Qlocation: 97, residue: Nlocation: 101, residue: Elocation: 104, residue: Elocation: 107, residue: Vlocation: 108, residue: Alocation: 128, residue: Vlocation: 131, residue: Ilocation: 172, residue: Ilocation: 201, residue: Rlocation: 226, residue: Alocation: 234, residue: Wlocation: 238, residue: Fputative receptor binding siteputative receptor binding site(16)location: 357, residue: Hlocation: 358, residue: Ylocation: 359, residue: Clocation: 360, residue: Ylocation: 361, residue: Plocation: 362, residue: Hlocation: 363, residue: Flocation: 364, residue: Tlocation: 365, residue: Clocation: 366, residue: Alocation: 367, residue: Vlocation: 368, residue: Dlocation: 370, residue: Elocation: 371, residue: Nlocation: 372, residue: Ilocation: 373, residue: RSwitch I regionSwitch I region(9)location: 199, residue: Rlocation: 200, residue: Clocation: 201, residue: Rlocation: 202, residue: Vlocation: 203, residue: Llocation: 204, residue: Tlocation: 205, residue: Slocation: 206, residue: Glocation: 207, residue: ISwitch II regionSwitch II region(17)location: 224, residue: Vlocation: 225, residue: Glocation: 226, residue: Alocation: 227, residue: Qlocation: 228, residue: Rlocation: 229, residue: Dlocation: 230, residue: Elocation: 231, residue: Rlocation: 232, residue: Rlocation: 233, residue: Klocation: 234, residue: Wlocation: 235, residue: Ilocation: 236, residue: Qlocation: 237, residue: Clocation: 238, residue: Flocation: 239, residue: Nlocation: 240, residue: DG1 boxG1 box(8)location: 47, residue: Glocation: 48, residue: Alocation: 49, residue: Glocation: 50, residue: Elocation: 51, residue: Slocation: 52, residue: Glocation: 53, residue: Klocation: 54, residue: NG2 boxG2 box(1)location: 204, residue: TG3 boxG3 box(4)location: 223, residue: Dlocation: 224, residue: Vlocation: 225, residue: Glocation: 226, residue: AG4 boxG4 box(4)location: 292, residue: Nlocation: 293, residue: Klocation: 294, residue: Qlocation: 295, residue: DG5 boxG5 box(3)location: 365, residue: Clocation: 366, residue: Alocation: 367, residue: VG-alphacd00066 (41-388): Alpha subunit of G proteins (guanine nucleotide binding)P-loop_NTPase superfamilycl38936(41-388): Members of the P-loop NTPase domain superfamily are characterized by a conserved nucleotide phosphate-binding motif, also referred to as the Walker A...
1
Guanine Nucleotide-binding Protein G(i)/g(s)/g(t) Subunit Beta-1
1 Protein
SS Diagram
3D Domains
Features
Domain Families
Specific Hits
Super Families
1100200300351351 residues, click to see VAST similar structures.helix 17-36strand 57-63strand 67-75strand 79-87strand 88-95strand 98-106strand 110-117strand 121-128strand 131-137strand 144-151strand 155-164strand 166-174strand 175-183strand 184-192strand 197-204strand 208-215strand 217-225strand 226-234strand 238-246strand 250-258strand 259-266strand 269-276strand 283-290strand 294-301strand 303-310strand 313-320strand 325-332strand 337-343strand 345-351Domain 1 (47-194): click to see VAST similar structures1Domain 2 (195-322): click to see VAST similar structures2Domain 1 (323-351): click to see VAST similar structures1structural tetradstructural tetrad(40)location: 65, residue: Hlocation: 83, residue: Slocation: 87, residue: Dlocation: 93, residue: Wlocation: 94, residue: Dlocation: 101, residue: Vlocation: 102, residue: Hlocation: 125, residue: Clocation: 129, residue: Dlocation: 135, residue: Ylocation: 136, residue: Nlocation: 152, residue: Glocation: 153, residue: Hlocation: 170, residue: Tlocation: 174, residue: Dlocation: 180, residue: Wlocation: 181, residue: Dlocation: 194, residue: Hlocation: 211, residue: Vlocation: 216, residue: Dlocation: 222, residue: Wlocation: 223, residue: Dlocation: 235, residue: Glocation: 236, residue: Hlocation: 254, residue: Tlocation: 258, residue: Dlocation: 264, residue: Flocation: 265, residue: Dlocation: 276, residue: Slocation: 277, residue: Hlocation: 297, residue: Llocation: 302, residue: Dlocation: 308, residue: Wlocation: 309, residue: Dlocation: 321, residue: Glocation: 322, residue: Hlocation: 340, residue: Tlocation: 344, residue: Dlocation: 350, residue: Wlocation: 351, residue: NWD40cd00200 (59-351): WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assemblyWD40 superfamilycl29593(59-351): WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal...
1
Guanine Nucleotide-binding Protein G(i)/g(s)/g(o) Subunit Gamma-2
1 Protein
SS Diagram
Features
Domain Families
Specific Hits
Super Families
1507171 residues, click to see VAST similar structures.helix 11-22helix 30-44beta subunit binding sitebeta subunit binding site(15)location: 9, residue: Ilocation: 12, residue: Alocation: 16, residue: Vlocation: 19, residue: Llocation: 23, residue: Alocation: 28, residue: Ilocation: 30, residue: Vlocation: 33, residue: Alocation: 34, residue: Alocation: 37, residue: Llocation: 38, residue: Mlocation: 41, residue: Clocation: 50, residue: Llocation: 51, residue: Llocation: 61, residue: FGGLsmart00224 (9-71): G protein gamma subunit-like motifsGGLcl00069(9-71): G protein gamma subunit-like motifs, the alpha-helical G-gamma chain dimerizes with the G-beta propeller subunit as part of the heterotrimeric G...
1
Nb35
1 Protein
SS Diagram
Features
Domain Families
Specific Hits
Super Families
1100162162 residues, click to see VAST similar structures.strand 26-32strand 33-38strand 39-50strand 56-64strand 69-77strand 81-85strand 91-99strand 100-111strand 115-123strand 145-151heterodimer interfaceheterodimer interface(6)location: 63, residue: Qlocation: 67, residue: Klocation: 71, residue: Wlocation: 119, residue: Ylocation: 142, residue: Rlocation: 143, residue: Gantigen binding siteantigen binding site(3)location: 57, residue: Klocation: 74, residue: Dlocation: 123, residue: Cintrachain IgC domain interfaceintrachain IgC domain interface(5)location: 33, residue: Glocation: 35, residue: Llocation: 147, residue: Qlocation: 149, residue: Tlocation: 151, residue: SIgV_Hcd04981 (27-151): Immunoglobulin (Ig) heavy chain (H), variable (V) domainIg superfamilycl11960(27-151): The members here are composed of the immunoglobulin (Ig) domain found in the Ig superfamily. The Ig superfamily is a heterogenous group of proteins,...
1
Soluble Cytochrome B562,trace Amine-associated Receptor 1
1 Protein
SS Diagram
3D Domains
Features
Domain Families
Specific Hits
Super Families
1100200300400510510 residues, click to see VAST similar structures.helix 145-174helix 182-199helix 203-211helix 219-251helix 263-286helix 295-304helix 313-323helix 326-354helix 374-388helix 389-398helix 408-419helix 435-447Domain 1 (1-290): click to see VAST similar structures1Domain 2 (309-427): click to see VAST similar structures2putative ligand binding siteputative ligand binding site(17)location: 224, residue: Hlocation: 228, residue: Dlocation: 229, residue: Ilocation: 232, residue: Slocation: 233, residue: Slocation: 309, residue: Vlocation: 316, residue: Glocation: 319, residue: Tlocation: 320, residue: Flocation: 323, residue: Slocation: 389, residue: Wlocation: 392, residue: Flocation: 393, residue: Flocation: 396, residue: Tlocation: 411, residue: Nlocation: 415, residue: Ilocation: 419, residue: YPRK15058PRK15058 (2-124): cytochrome b562Cytochrom_B562cl01546(2-124): This family contains the bacterial cytochrome b562. This forms a four-helix bundle that non-covalently binds a single heme prosthetic group..7tmA_TAAR1cd15314 (151-440): trace amine-associated receptor 1 and similar receptors, member of the class A family of seven-transmembrane G protein-coupled receptors7tm_GPCRs superfamilycl28897(151-440): This hierarchical evolutionary model represents the seven-transmembrane (7TM) receptors, often referred to as G protein-coupled receptors (GPCRs),...
Chemical and Non-standard biopolymers (1 molecule)
1
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