| Run | Spots | Bases | Size | GC content | Published | Access Type |
|---|---|---|---|---|---|---|
DRR045555 |
63.1M | 6.4Gbp | 4.0G | 50% | 2016-05-09 | public |
This run has 1 read per spot:
| L=101, 100% |
- Legend
Technical read Application Read L=4, 100% Length is 4, 100% spots contain this read ̅L=165, σ=92.8, 66% Average length is 165, standard deviation is 92.8, 66% spots contain this read
| Experiment | Library Name | Platform | Strategy | Source | Selection | Layout | Action |
|---|---|---|---|---|---|---|---|
| DRX040851 | Illumina | RNA-Seq | TRANSCRIPTOMIC | cDNA | SINGLE |
| Biosample | Sample Description | Organism |
|---|---|---|
| SAMD00040042 (DRS029617) | Heterocephalus glaber |
| Bioproject | SRA Study | Title |
|---|---|---|
| PRJDB4191 | DRP003111 | RNA sequence of Naked mole rat induced pluripotent stem cells. |
We have generated induced pluripotent stem cells (iPSCs) from naked mole rat (NMR, Heterocephalus glaber) using retrovirus-mediated delivery of reprogramming factors (mouse Oct4, Sox2, Klf4 and c-Myc). NMR-iPSCs are cultured on mitomycin C-treated SNL-STO feeder cells. Although NMR-iPSCs exhibit pluripotency (the potential to differentiate into cells of the three germ layers) in vitro, they do not form teratomas in vivo. The expression of the tumour suppressor alternative reading frame (ARF) is activated in NMR-iPSCs compared with fibroblasts. NMRs have a unique frame-shift mutation in transforming-oncogene ES cell-expressed Ras (ERAS) that positively regulates tumourigenicity of mouse ESCs. To demonstrate the role of ARF activation and ERAS disruption in the tumour resistance of NMR-iPSCs, we introduced short hairpin RNA targeting ARF (shARF) and/or mouse-ERas (mERas) into NMR-iPSCs (shARF-, mERas-, or shARF/mERas-NMR-iPSCs). NMR-iPSCs acquired tumorigenicity to form teratom a by introducing shARF and mERas. Submitted overall design: The data is deep sequencing of mRNA from NMR-iPSCs (four clones: NMR-iPS 4, 10, 24 and 27), NMR skin fibroblast (two lines: H5 and H17) and NMR-iPSCs clone 24 and 27 introduced sh ARF and/or mERas (shARF-, mERas-, or shARF/mERas-NMR-iPSCs). Gene expression profiles of these cell lines were analyzed.
SRA archive data
SRA archive data is normalized by the SRA load process and used by the SRA Toolkit to read and produce formats like FASTQ, SAM, etc. The default toolkit configuration enables it to find and retrieve SRA runs by accession.
Public SRA files are now available from GCP and AWS cloud platforms as well as from NCBI. Access to most data in the cloud requires a user account with the cloud service provider. The user’s account will incur costs for cloud compute or to copy data outside of the specified cloud service region.
| Type | Size | Location | Name | Free Egress | Access Type |
|---|---|---|---|---|---|
| SRA Normalized | 3,918,836 Kb | AWS | https://sra-pub-run-odp.s3.amazonaws.com/sra/DRR045555/DRR045555 | worldwide | anonymous |
| SRA Lite | 1,680,563 Kb | NCBI | https://sra-downloadb.be-md.ncbi.nlm.nih.gov/sos4/sra-pub-zq-5/DRR000/045/DRR045555/DRR045555.sralite.1 | worldwide | anonymous |
| AWS | s3://sra-pub-zq-7/DRR045555/DRR045555.sralite.1 | s3.us-east-1 | aws identity | ||
| GCP | gs://sra-pub-zq-101/DRR045555/DRR045555.zq.1 | gs.us-east1 | gcp identity |
- Egress and Access: what does it mean?
-
Egress
term used by cloud providers to describe cost (charged to the user) of moving data outside of the storage region Free Egresscolumn indicates where the data can be accessed without an egress charge: - worldwide can be downloaded from anywhere for free
- s3.us-east-1 is free to access from machines running in Amazon's us-east-1 region, access from other regions or transport outside of AWS will require paying egress charges
- gs.US is free to access from machines running in Google’s gs.US region, access from other regions or transport outside of GCP will require paying egress charges
Access Typedescribes whether a cloud service user account is necessary for data access. "anonymous" access means general public access Namecolumn provides either a link to free download location at NCBI or a URL for the cloud provider storage location. Either s3:// for Amazon or gs:// for Google storage
- Why is SRA data in the cloud?
-
In order to support large scale (hyper parallel) data analyses SRA data is now available at GCP and AWS with few caveats:
- SRA data is copied to the cloud from NCBI. There may be a lag between availability from NCBI and from CSP (cloud service providers)
- To access public data user account with the cloud service provider is required. Your account will incur costs for cloud compute and/or to copy data (either archival or results of your comute) outside of the specified cloud service region
- Distribution of protected data is signed by NIH account and requires user to operate in the same region as the data
SRA has also begun to provide access to originally submitted source files:
- not all files have been validated by SRA
- not all files have been copied to cloud locations (recovering it from NCBI tape system takes time ).
- the volume of this type of data a much larger and it is not used as often so we will keep most of it on tape or "cold" storage in cloud. As a result the data may not be available instantly and restore requests will be served on first-come first-served basis and cost of resore may be charged to your user account.
- What is "Cloud Data Delivery"?
-
Please visit SRA Data Delivery service to request Sequence Read Archive (SRA) data to be delivered to an Amazon Web Services (AWS) or Google Cloud Platform (GCP) bucket of your choice.