| Run | Spots | Bases | Size | GC content | Published | Access Type |
|---|---|---|---|---|---|---|
SRR060015 |
33.5k | 9.0Mbp | 19.3M | 42% | 2011-02-01 | public |
This run has 2 reads per spot:
| L=4, 100% | ̅L=264, σ=30.9, 100% |
- Legend
Technical read Application Read L=4, 100% Length is 4, 100% spots contain this read ̅L=165, σ=92.8, 66% Average length is 165, standard deviation is 92.8, 66% spots contain this read
| Experiment | Library Name | Platform | Strategy | Source | Selection | Layout | Action |
|---|---|---|---|---|---|---|---|
| SRX023385 | wq_heads_FLX | LS454 | EST | TRANSCRIPTOMIC | unspecified | SINGLE |
Design:
| Biosample | Sample Description | Organism |
|---|---|---|
| SAMN00016997 (SRS084970) | The heads of ~100 workers and queens from five nests collected in Gainesville Florida were combined into a single pool and total RNA was extracted using Trizol (Invitrogen). Total mRNA was isolated using the OligoDirect mini mRNA extraction kit following the manufacturer’s suggested protocol (Qiagen). Double stranded cDNA libraries were constructed using the SMART cDNA synthesis kit (Clontech) following the manufacturer’s suggested protocol. Approximately 500 nanograms of mRNA was used as starting material. Each of the four cDNA libraries were then normalized using the TRIMMER kit (Evrogen). | Solenopsis invicta |
| Bioproject | SRA Study | Title |
|---|---|---|
| PRJNA49629 | SRP002592 | Solenopsis invicta genome sequencing project |
A 454 shotgun sequencing library and an Illumina 330bp-insert paired-end library were built from the DNA of the single focal haploid male. These libraries made up 93.8% of our sequencing effort. The focal male had the Gp-9 B genotype. He was the son of a queen from a multiple-queen colony originally collected near Athens, Georgia, USA in 2008. Upon transfer of the colony to the laboratory, the queen had been isolated with workers for one year to ensure that all progeny in the colony were hers. To permit bridging of repeats that could be longer than the 454 read length, we additionally constructed 8kb and 20kb-insert paired-end 454 libraries for the remaining 6.2% of sequencing effort. The 8kb and 20kb libraries were respectively constructed from DNA pooled from ten and thirty-one brothers of the focal male, all having the Gp-9 B genotype.
SRA archive data
SRA archive data is normalized by the SRA load process and used by the SRA Toolkit to read and produce formats like FASTQ, SAM, etc. The default toolkit configuration enables it to find and retrieve SRA runs by accession.
Public SRA files are now available from GCP and AWS cloud platforms as well as from NCBI. Access to most data in the cloud requires a user account with the cloud service provider. The user’s account will incur costs for cloud compute or to copy data outside of the specified cloud service region.
| Type | Size | Location | Name | Free Egress | Access Type |
|---|---|---|---|---|---|
| SRA Normalized | 18,892 Kb | AWS | https://sra-pub-run-odp.s3.amazonaws.com/sra/SRR060015/SRR060015 | worldwide | anonymous |
| SRA Lite | 16,453 Kb | NCBI | https://sra-downloadb.be-md.ncbi.nlm.nih.gov/sos5/sra-pub-zq-11/SRR000/060/SRR060015/SRR060015.lite.1 | worldwide | anonymous |
| AWS | s3://sra-pub-zq-9/SRR060015/SRR060015.lite.1 | s3.us-east-1 | aws identity | ||
| GCP | gs://sra-pub-zq-109/SRR060015/SRR060015.lite.1 | gs.us-east1 | gcp identity |
Original format
The original files submitted to SRA. These files may require specific software to open, read and interpret data.
| Type | Size | Location | Name | Free Egress | Access Type |
|---|---|---|---|---|---|
| sff | 54,363 Kb | AWS | s3://sra-pub-src-9/SRR060015/female_FLX.sff | - | Use Cloud Data Delivery |
- Egress and Access: what does it mean?
-
Egress
term used by cloud providers to describe cost (charged to the user) of moving data outside of the storage region Free Egresscolumn indicates where the data can be accessed without an egress charge: - worldwide can be downloaded from anywhere for free
- s3.us-east-1 is free to access from machines running in Amazon's us-east-1 region, access from other regions or transport outside of AWS will require paying egress charges
- gs.US is free to access from machines running in Google’s gs.US region, access from other regions or transport outside of GCP will require paying egress charges
Access Typedescribes whether a cloud service user account is necessary for data access. "anonymous" access means general public access Namecolumn provides either a link to free download location at NCBI or a URL for the cloud provider storage location. Either s3:// for Amazon or gs:// for Google storage
- Why is SRA data in the cloud?
-
In order to support large scale (hyper parallel) data analyses SRA data is now available at GCP and AWS with few caveats:
- SRA data is copied to the cloud from NCBI. There may be a lag between availability from NCBI and from CSP (cloud service providers)
- To access public data user account with the cloud service provider is required. Your account will incur costs for cloud compute and/or to copy data (either archival or results of your comute) outside of the specified cloud service region
- Distribution of protected data is signed by NIH account and requires user to operate in the same region as the data
SRA has also begun to provide access to originally submitted source files:
- not all files have been validated by SRA
- not all files have been copied to cloud locations (recovering it from NCBI tape system takes time ).
- the volume of this type of data a much larger and it is not used as often so we will keep most of it on tape or "cold" storage in cloud. As a result the data may not be available instantly and restore requests will be served on first-come first-served basis and cost of resore may be charged to your user account.
- What is "Cloud Data Delivery"?
-
Please visit SRA Data Delivery service to request Sequence Read Archive (SRA) data to be delivered to an Amazon Web Services (AWS) or Google Cloud Platform (GCP) bucket of your choice.
Taxonomy Analysis
- Unidentified reads: 12.94%
- Identified reads: 87.06%
Strong signals
| SuperKingdom | Organism | Rank | %% | Kbp | Coverage |
|---|---|---|---|---|---|
| Eukaryota | Solenopsis invicta | species | 87.0 | 7,824 |
- How to read results?
Results show distribution of reads mapping to specific taxonomy nodes as a percentage of total reads within the analyzed run. In cases where a read maps to more than one related taxonomy node, the read is reported as originating from the lowest shared taxonomic node. So when a read maps to two species belonging to the same genus, it is assigned at the genus level. Sequence reads from a single organism will map to several taxonomy nodes spanning the organism’s lineage. The number of reads mapping to higher level nodes will typically be greater than those that map to terminal nodes.
STAT results are proportional to the size of sequenced genomes. Given a mixed sample containing several organisms at equal copy number, proportionally more reads originate from the larger genomes. This means that the percentages reported by STAT will reflect genome size and must be considered against the genomic complexity of the sequenced sample.
- How can I query this analysis data?
Please take a look at NCBI SRA Cloud-based Taxonomy Analysis Table