Run Browser : Browse : Sequence Read Archive : NCBI/NLM/NIH

Run	Spots	Bases	Size	GC content	Published	Access Type
SRR5270353	54.8M	9.7Gbp	6.9G	51.9%	2017-02-28	public

Quality graph (bigger)(smaller)

241

Phred quality score

This run has 2 reads per spot:

̅L=92, σ=17.1, 99%

̅L=93, σ=16.4, 93%

Legend

		Technical read
		Application Read
L=4, 100%		Length is 4, 100% spots contain this read
̅L=165, σ=92.8, 66%		Average length is 165, standard deviation is 92.8, 66% spots contain this read

Experiment	Library Name	Platform	Strategy	Source	Selection	Layout	Action
SRX2574408	SAp1	Illumina	RNA-Seq	TRANSCRIPTOMIC	RANDOM	PAIRED

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Salivary glands (SG) were dissected from engorged female ticks collected from the infected and uninfected dogs and stored in RNAlater (Ambion) at -20ºC. Total RNA from the SG was extracted using the All-in-One Purification Kit (Norgen Biotek Corporation), following the manufacturer’s instructions. RNA concentration and integrity were evaluated by analysis of rRNA integrity using Agilent 2100 Bioanalyzer (Agilent Technologies Inc.). Libraries preparation was performed with "NEBNext Ultra Directional RNA Library Prep" kit (New England Biolabs) following manufacturer’s instructions. Briefly, magnetic beads with Oligo(dT) were used to enrich poly (A) mRNA from 1 µg of total RNA. Next, the purified mRNA was disrupted into short fragments, and double-stranded cDNA was immediately synthesised. cDNA was subjected to end-repair and adenylation, then connected with sequencing adapters. Suitable fragments, purified by size selection protocol with AMPure XP beads (Beckman Coulter), were selected as templates for PCR amplification. The final library sizes and qualities were evaluated electrophoretically using an Agilent High Sensitivity DNA kit (Agilent Technologies); the fragment size range was 300–1300 bp. Subsequently, the library was sequenced using a HiSeq 2500 sequencer (Illumina) in rapid run mode. Cluster generation was performed, followed by 2x100 cycle sequencing reads separated by a paired-end turnaround. Image analysis was performed using the HiSeq control software version 1.8.4.

Biosample	Sample Description	Organism
SAMN06242557 (SRS1959672)	E. canis Jaboticabal strain	Rhipicephalus sanguineus

Bioproject	SRA Study	Title
PRJNA362595	SRP098920	Rhipicephalus sanguineus sialotranscriptome

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This study concerns of a transcriptomic analysis of salivary glands of adult female Rhipicephalus sanguineus in response to Ehrlichia canis infection. Such information can be used for the selection of potential targets to develop anti-tick and pathogen transmission blocking vaccines.

SRA archive data

SRA archive data is normalized by the SRA load process and used by the SRA Toolkit to read and produce formats like FASTQ, SAM, etc. The default toolkit configuration enables it to find and retrieve SRA runs by accession.

Public SRA files are now available from GCP and AWS cloud platforms as well as from NCBI. Access to most data in the cloud requires a user account with the cloud service provider. The user’s account will incur costs for cloud compute or to copy data outside of the specified cloud service region.

Type	Size	Location	Name	Free Egress	Access Type
SRA Normalized	6,736,916 Kb	AWS	https://sra-pub-run-odp.s3.amazonaws.com/sra/SRR5270353/SRR5270353	worldwide	anonymous
SRA Lite	2,549,078 Kb	NCBI	https://sra-downloadb.be-md.ncbi.nlm.nih.gov/sos5/sra-pub-zq-14/SRR005/270/SRR5270353.sralite.1	worldwide	anonymous
		AWS	s3://sra-pub-zq-4/SRR5270353/SRR5270353.sralite.1	s3.us-east-1	aws identity
		GCP	gs://sra-pub-zq-102/SRR5270353/SRR5270353.zq.1	gs.us-east1	gcp identity

Egress and Access: what does it mean?

Egressterm used by cloud providers to describe cost (charged to the user) of moving data outside of the storage region

Free Egress column indicates where the data can be accessed without an egress charge:

worldwide can be downloaded from anywhere for free
s3.us-east-1 is free to access from machines running in Amazon's us-east-1 region, access from other regions or transport outside of AWS will require paying egress charges
gs.US is free to access from machines running in Google’s gs.US region, access from other regions or transport outside of GCP will require paying egress charges

Access Typedescribes whether a cloud service user account is necessary for data access. "anonymous" access means general public access

Namecolumn provides either a link to free download location at NCBI or a URL for the cloud provider storage location. Either s3:// for Amazon or gs:// for Google storage

Why is SRA data in the cloud?

In order to support large scale (hyper parallel) data analyses SRA data is now available at GCP and AWS with few caveats:

SRA data is copied to the cloud from NCBI. There may be a lag between availability from NCBI and from CSP (cloud service providers)
To access public data user account with the cloud service provider is required. Your account will incur costs for cloud compute and/or to copy data (either archival or results of your comute) outside of the specified cloud service region
Distribution of protected data is signed by NIH account and requires user to operate in the same region as the data

SRA has also begun to provide access to originally submitted source files:

not all files have been validated by SRA
not all files have been copied to cloud locations (recovering it from NCBI tape system takes time ).
the volume of this type of data a much larger and it is not used as often so we will keep most of it on tape or "cold" storage in cloud. As a result the data may not be available instantly and restore requests will be served on first-come first-served basis and cost of resore may be charged to your user account.

What is "Cloud Data Delivery"?: Please visit SRA Data Delivery service to request Sequence Read Archive (SRA) data to be delivered to an Amazon Web Services (AWS) or Google Cloud Platform (GCP) bucket of your choice.

Taxonomy Analysis

Unidentified reads: 93.75%
Identified reads: 6.25%

View in Krona

Strong signals

SuperKingdom	Organism	Rank	%%	Kbp
Eukaryota	Ixodes scapularis	species	6.0	607,710
Eukaryota	Amniota		0.1	13,784
Eukaryota	Actinopterygii	superclass	0.1	6,185
Bacteria	Actinobacteria	class	0.1	5,919
Bacteria	Mesorhizobium	genus	0.0	2,176
Eukaryota	Stramenopiles		0.0	2,100
Bacteria	Pseudomonas	genus	0.0	476
Bacteria	Comamonadaceae	family	0.0	398
Bacteria	Rhizobiaceae	family	0.0	355

How to read results?: Results show distribution of reads mapping to specific taxonomy nodes as a percentage of total reads within the analyzed run. In cases where a read maps to more than one related taxonomy node, the read is reported as originating from the lowest shared taxonomic node. So when a read maps to two species belonging to the same genus, it is assigned at the genus level. Sequence reads from a single organism will map to several taxonomy nodes spanning the organism’s lineage. The number of reads mapping to higher level nodes will typically be greater than those that map to terminal nodes.

STAT results are proportional to the size of sequenced genomes. Given a mixed sample containing several organisms at equal copy number, proportionally more reads originate from the larger genomes. This means that the percentages reported by STAT will reflect genome size and must be considered against the genomic complexity of the sequenced sample.

How can I query this analysis data?: Please take a look at NCBI SRA Cloud-based Taxonomy Analysis Table

How taxonomy analysis is done?: A fast, scalable, MinHash-based k-mer tool to assess Sequence Read Archive next generation sequence submissions

RNA-seq of salivary glands from Rhipicephalus sanguineus infected with Ehrlichia canis: Sap1(SRR5270353)

SRA archive data

Taxonomy Analysis

Strong signals

Intensity graph

	View: biological readstechnical readsquality scoresadvanced options Hide Reads base space color space show separated reads quality score with bases show clipped show 4 channel quality scorewith bases Intensity graph Signals table
	Intensity graph