Run Browser : Browse : Sequence Read Archive : NCBI/NLM/NIH

Run	Spots	Bases	Size	GC content	Published	Access Type
SRR9678467	34.0M	10.2Gbp	4.3G	46.9%	2020-06-10	public

Quality graph (bigger)(smaller)

241

Phred quality score

This run has 2 reads per spot:

L=150, 100%

Legend

		Technical read
		Application Read
L=4, 100%		Length is 4, 100% spots contain this read
̅L=165, σ=92.8, 66%		Average length is 165, standard deviation is 92.8, 66% spots contain this read

Experiment	Library Name	Platform	Strategy	Source	Selection	Layout	Action
SRX6438742	w501_male_brain_rep2_L4	Illumina	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED

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Prior to the dissections, all fly lines were expanded and maintained at 24C under an undisturbed 12:12 light/dark cycle in low density vials with fly food. All individuals were mated and precisely at 4-days-old. Flies were very briefly anesthetized with CO2 and heads were collected with a clean razor blade. Heads were transferred to a glass dissection plate with PBS for brain collection. Dissections were performed within a 3-hour window always at the same circadian zeitgeber time (ZT1-ZT4; in this experiment the light was turned on at 8 am, and the dissections were performed between 9 am and 12 pm within a three-hour interval). Male and female brains of the same line were dissected in parallel the same day at the same time. Dissected brains were immediately transferred into a low retention Eppendorf tube containing 100 mL Trizol (Invitrogen) and kept frozen at -20C until RNA extraction. We collected 3 biological replicates for each genotype by sex combination, and each replicate comprised of a pool of exactly 15 brains.

Biosample	Sample Description	Organism	Links
SAMN12261923 (SRS5093406)		Drosophila simulans	PRJNA553234

Bioproject	SRA Study	Title
PRJNA553234	SRP214528	Transcriptomes of the brain of Drosophila species subgroup

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In this study, we generated and analyzed population scale gene expression data from female and male brains of several strains of Drosophila melanogaster, D. simulans, and D. yakuba to answer questions on gene expression evolution, gene origination, and genome evolution.

SRA archive data

SRA archive data is normalized by the SRA load process and used by the SRA Toolkit to read and produce formats like FASTQ, SAM, etc. The default toolkit configuration enables it to find and retrieve SRA runs by accession.

Public SRA files are now available from GCP and AWS cloud platforms as well as from NCBI. Access to most data in the cloud requires a user account with the cloud service provider. The user’s account will incur costs for cloud compute or to copy data outside of the specified cloud service region.

Type	Size	Location	Name	Free Egress	Access Type
SRA Normalized	4,164,624 Kb	AWS	https://sra-pub-run-odp.s3.amazonaws.com/sra/SRR9678467/SRR9678467	worldwide	anonymous
SRA Lite	2,497,259 Kb	NCBI	https://sra-downloadb.be-md.ncbi.nlm.nih.gov/sos3/sra-pub-zq-24/SRR009/9678/SRR9678467/SRR9678467.lite.1	worldwide	anonymous
		AWS	s3://sra-pub-zq-8/SRR9678467/SRR9678467.lite.1	s3.us-east-1	aws identity
		GCP	gs://sra-pub-zq-103/SRR9678467/SRR9678467.lite.1	gs.us-east1	gcp identity

Original format

The original files submitted to SRA. These files may require specific software to open, read and interpret data.

Type	Size	Location	Name	Free Egress	Access Type
fastq	2,783,251 Kb	AWS	s3://sra-pub-src-14/SRR9678467/w501mBr_b_D08L4_1.fq.gz.1	-	Use Cloud Data Delivery
fastq	3,280,007 Kb	AWS	s3://sra-pub-src-14/SRR9678467/w501mBr_b_D08L4_2.fq.gz.1	-	Use Cloud Data Delivery

Egress and Access: what does it mean?

Egressterm used by cloud providers to describe cost (charged to the user) of moving data outside of the storage region

Free Egress column indicates where the data can be accessed without an egress charge:

worldwide can be downloaded from anywhere for free
s3.us-east-1 is free to access from machines running in Amazon's us-east-1 region, access from other regions or transport outside of AWS will require paying egress charges
gs.US is free to access from machines running in Google’s gs.US region, access from other regions or transport outside of GCP will require paying egress charges

Access Typedescribes whether a cloud service user account is necessary for data access. "anonymous" access means general public access

Namecolumn provides either a link to free download location at NCBI or a URL for the cloud provider storage location. Either s3:// for Amazon or gs:// for Google storage

Why is SRA data in the cloud?

In order to support large scale (hyper parallel) data analyses SRA data is now available at GCP and AWS with few caveats:

SRA data is copied to the cloud from NCBI. There may be a lag between availability from NCBI and from CSP (cloud service providers)
To access public data user account with the cloud service provider is required. Your account will incur costs for cloud compute and/or to copy data (either archival or results of your comute) outside of the specified cloud service region
Distribution of protected data is signed by NIH account and requires user to operate in the same region as the data

SRA has also begun to provide access to originally submitted source files:

not all files have been validated by SRA
not all files have been copied to cloud locations (recovering it from NCBI tape system takes time ).
the volume of this type of data a much larger and it is not used as often so we will keep most of it on tape or "cold" storage in cloud. As a result the data may not be available instantly and restore requests will be served on first-come first-served basis and cost of resore may be charged to your user account.

What is "Cloud Data Delivery"?: Please visit SRA Data Delivery service to request Sequence Read Archive (SRA) data to be delivered to an Amazon Web Services (AWS) or Google Cloud Platform (GCP) bucket of your choice.

Taxonomy Analysis

Unidentified reads: 0.36%
Identified reads: 99.64%

View in Krona

Strong signals

SuperKingdom	Organism	Rank	%%	Kbp	Coverage
Eukaryota	Drosophila simulans	species	99.6	10,145,158	103.6
Bacteria	Acinetobacter baumannii	species	0.0	4,130	1.0
Bacteria	Burkholderiales	order	0.0	2,719
Bacteria	Alphaproteobacteria	class	0.0	1,433
Bacteria	Terrabacteria group		0.0	598
Bacteria	Xanthomonadales	order	0.0	404
Viruses	Alfalfa mosaic virus	species	0.0	2

How to read results?: Results show distribution of reads mapping to specific taxonomy nodes as a percentage of total reads within the analyzed run. In cases where a read maps to more than one related taxonomy node, the read is reported as originating from the lowest shared taxonomic node. So when a read maps to two species belonging to the same genus, it is assigned at the genus level. Sequence reads from a single organism will map to several taxonomy nodes spanning the organism’s lineage. The number of reads mapping to higher level nodes will typically be greater than those that map to terminal nodes.

STAT results are proportional to the size of sequenced genomes. Given a mixed sample containing several organisms at equal copy number, proportionally more reads originate from the larger genomes. This means that the percentages reported by STAT will reflect genome size and must be considered against the genomic complexity of the sequenced sample.

How can I query this analysis data?: Please take a look at NCBI SRA Cloud-based Taxonomy Analysis Table

How taxonomy analysis is done?: A fast, scalable, MinHash-based k-mer tool to assess Sequence Read Archive next generation sequence submissions

RNA-sequencing of Drosophila simulans adult male brain(SRR9678467)

SRA archive data

Original format

Taxonomy Analysis

Strong signals

Intensity graph

	View: biological readstechnical readsquality scoresadvanced options Hide Read base space color space show separated reads quality score with bases show clipped show 4 channel quality scorewith bases Intensity graph Signals table
	Intensity graph