Spatially varying cis-regulatory divergence in Drosophila embryos elucidates cis-regulatory logic
Study Type:
Other
Abstract: Both cis- and trans-acting changes could accumulate and participate in complex interactions, so to isolate the cis-regulatory component of patterning evolution, we measured allele-specific spatial gene expression patterns in Drosophila melanogaster × D. simulans hybrid embryos. RNA-seq of cryosectioned slices revealed 55 genes with strong spatially-varying allele-specific expression, and several hundred more with weaker but significant spatial divergence. Combined with mathematical modeling and regulatory locus editing, we determine the SNP responsible for the allele-specific expression observed in the gene hunchback. Overall design: Embryonic mRNA profiles of mid-stage 5 hybrid and parental embryos were generated using RNA-seq
Center Project: GSE102233
External Link: /pubmed:30383747
| Organism | Sample | File Name | Size | Updated |
|---|---|---|---|---|
| Drosophila melanogaster x Drosophila simulans | SAMN07447695 | simXmel_cyc14C_rep1_sl22_R1_run1.fastq.gz | 265.1Mb | 2020-03-23 04:25:51 |
| Drosophila melanogaster x Drosophila simulans | SAMN07447695 | simXmel_cyc14C_rep1_sl22_R2_run1.fastq.gz | 274.1Mb | 2020-03-22 21:59:29 |