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IDs: 5334631 [UID] 15441038 [GenBank] 15648578 [RefSeq]
The assembly mSarHar1.11 is based on 88x ONT data including 10x ultra long reads sequenced at Oxford Nanopore Technologies; 60x 10X Genomics Chromium data, BioNano data, 50x Illumina HiSeq XTen and 60x Dovetail Hi-C data generated at the Wellcome ... Sanger Institute. The assembly process included the following sequence of steps: initial ONT assembly generation with WTDBG, 10X based scaffolding with scaff10x, BioNano hybrid-scaffolding, Hi-C based scaffolding with scaffHiC, WTDBG2/Racon polishing, and two rounds of FreeBayes polishing. Finally, the assembly was analysed and manually improved using gEVAL, where haplotigs have been removed. Chromosome-scale scaffolds have been confirmed using the Hi-C data. Chromosomes are named according to established convention and the labels for chromosomes 1 and 2 are switched compared with a previous genome assembly (Devil7.0) (i.e. the chromosome labelled 1 in Devil7.0 is labelled 2 in the current assembly, and vice versa). Two contigs derived from the Y chromosome from a different individual, and sequenced with Illumina HiSeq 2000, have been added. The MT sequence from Devil7.0 has also been included. more
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