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IDs: 1529631 [UID] 5907498 [GenBank] 6026018 [RefSeq]
This is the whole-genome assembly of a female orangutan, Susie. Genome assembly was done in stages, incorporating data from multiple genomic platforms including PacBio, Illumina, and BioNano optical maps, resulting in three progressive versions: V0 (GCA_002880775.1): The backbone of the ... assembly is made up of contigs obtained by assembling the PacBio (P6-C4 chemistry) reads using the Falcon assembler. These contigs are error-corrected using the tool Quiver and then further error-corrected using Pilon with 37-fold Illumina paired-end reads. V1 (GCA_002880775.2): BioNano optical maps are used to place the contigs into scaffolds, followed by chromosome-level scaffolding using bicolor FISH. V2 (GCA_002880775.3): The assembly is further error-corrected, particularly in regions that have clustered 1-2 bp indels. This is done by applying an Eichler-lab-created Freebayes-based indel correction method. more
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