rock2b rho-associated, coiled-coil containing protein kinase 2b [Danio rerio (zebrafish)] - Gene

Summary

Official Symbol: rock2bprovided by ZNC
Official Full Name: rho-associated, coiled-coil containing protein kinase 2bprovided by ZNC
Primary source: ZFIN:ZDB-GENE-060125-2
See related: Ensembl:ENSDARG00000004877 AllianceGenome:ZFIN:ZDB-GENE-060125-2
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Danio rerio
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; Danionidae; Danioninae; Danio
Also known as: cb64; sb:cb64; si:dkey-65b13.7; si:dkey-65b13.10
Summary: Predicted to enable Rho-dependent protein serine/threonine kinase activity. Acts upstream of or within Kupffer's vesicle development; epithelial cilium movement involved in determination of left/right asymmetry; and heart morphogenesis. Predicted to be located in plasma membrane. Predicted to be integral component of membrane. Predicted to be active in centrosome and cytoplasm. Is expressed in several structures, including EVL; Kupffer's vesicle; forerunner cell group; hindbrain; and somite. Orthologous to human ROCK2 (Rho associated coiled-coil containing protein kinase 2). [provided by Alliance of Genome Resources, Apr 2022]
Orthologs: all
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Genomic context

Location:: chromosome: 20

Exon count:: 42

Annotation release	Status	Assembly	Chr	Location
106	current	GRCz11 (GCF_000002035.6)	20	NC_007131.7 (33575452..33705230, complement)
105	previous assembly	GRCz10 (GCF_000002035.5)	20	NC_007131.6 (33672573..33804146, complement)

Chromosome 20 - NC_007131.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
Description: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
BioProject: PRJEB1986
Analysis date: Fri Dec 8 19:48:10 2017

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Antisense depletion of rock2b in the whole embryo or specifically in the KV cell lineage perturbed asymmetric gene expression in lateral plate mesoderm and disrupted organ LR asymmetries.
Title: The Rho kinase Rock2b establishes anteroposterior asymmetry of the ciliated Kupffer's vesicle in zebrafish.

Submit: New GeneRIF Correction

General gene information

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Markers

fj66e11.x1 (e-PCR)
- UniSTS:199797

fc90c03.y1 (e-PCR)
- UniSTS:194780

Gene Ontology Provided by ZFIN

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables Rho-dependent protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables small GTPase binding	IEA Inferred from Electronic Annotation more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within Kupffer's vesicle development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in Rho protein signal transduction	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within Rho protein signal transduction	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within actin cytoskeleton organization	IEA Inferred from Electronic Annotation more info
involved_in actomyosin structure organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in cortical actin cytoskeleton organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in embryonic morphogenesis	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within epithelial cilium movement involved in determination of left/right asymmetry	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within heart morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic cytokinesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in peptidyl-threonine phosphorylation	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within positive regulation of centrosome duplication	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of actin cytoskeleton organization	IEA Inferred from Electronic Annotation more info
involved_in regulation of cell junction assembly	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within smooth muscle contraction	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in centrosome	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytoskeleton	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: rho-associated protein kinase 2b; rho-associated, coiled-coil containing protein kinase 2b

Names: rho-kinase 2

NP_001087216.1

EC 2.7.11.1

XP_009292970.1

EC 2.7.11.1

XP_009292972.1

EC 2.7.11.1

XP_017207922.1

EC 2.7.11.1

XP_017207923.1

EC 2.7.11.1

XP_017207926.1

EC 2.7.11.1

XP_021324031.1

EC 2.7.11.1

XP_021324032.1

EC 2.7.11.1

XP_021324033.1

EC 2.7.11.1

XP_021324034.1

EC 2.7.11.1

XP_021324035.1

EC 2.7.11.1

XP_021324036.1

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001093747.2 → NP_001087216.1 rho-associated protein kinase 2b

See identical proteins and their annotated locations for NP_001087216.1

Status: PROVISIONAL

Source sequence(s)

AL929220, BX571790

UniProtKB/TrEMBL

A0A8M1NDD8, F1R417

Conserved Domains (7) summary

smart00220
Location:78 → 340

S_TKc; Serine/Threonine protein kinases, catalytic domain

COG1196
Location:432 → 1097

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

cd00029
Location:1253 → 1307

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd01242
Location:1145 → 1249

PH_ROCK; Rho-associated coiled-coil containing protein kinase pleckstrin homology (PH) domain

cd05596
Location:51 → 402

STKc_ROCK; Catalytic domain of the Serine/Threonine Kinase, Rho-associated coiled-coil containing protein kinase

pfam08912
Location:967 → 1034

Rho_Binding; Rho Binding

cl00087
Location:494 → 560

HR1; Protein kinase C-related kinase homology region 1 (HR1) domain that binds Rho family small GTPases

RefSeqs of Annotated Genomes: Danio rerio Annotation Release 106 details...

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCz11 Primary Assembly

Genomic

NC_007131.7 Reference GRCz11 Primary Assembly

Range

33575452..33705230 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_021468360.1 → XP_021324035.1 rho-associated, coiled-coil containing protein kinase 2b isoform X6

UniProtKB/TrEMBL

A0A8M9PSI1

Conserved Domains (3) summary

cd05596
Location:51 → 402

STKc_ROCK; Catalytic domain of the Serine/Threonine Kinase, Rho-associated coiled-coil containing protein kinase

cl21453
Location:1228 → 1413

PKc_like; Protein Kinases, catalytic domain

cl25732
Location:432 → 1097

SMC_N; RecF/RecN/SMC N terminal domain
XM_021468361.1 → XP_021324036.1 rho-associated, coiled-coil containing protein kinase 2b isoform X8

UniProtKB/TrEMBL

A0A8M9PP57

Conserved Domains (3) summary

cd05596
Location:51 → 402

STKc_ROCK; Catalytic domain of the Serine/Threonine Kinase, Rho-associated coiled-coil containing protein kinase

cl21453
Location:1161 → 1346

PKc_like; Protein Kinases, catalytic domain

cl25732
Location:432 → 1097

SMC_N; RecF/RecN/SMC N terminal domain
XM_021468359.1 → XP_021324034.1 rho-associated, coiled-coil containing protein kinase 2b isoform X3

UniProtKB/TrEMBL

A0A8M9PH50

Conserved Domains (4) summary

cd00029
Location:1320 → 1374

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd01242
Location:1212 → 1316

PH_ROCK; Rho-associated coiled-coil containing protein kinase pleckstrin homology (PH) domain

cd05596
Location:51 → 402

STKc_ROCK; Catalytic domain of the Serine/Threonine Kinase, Rho-associated coiled-coil containing protein kinase

cl25732
Location:432 → 1097

SMC_N; RecF/RecN/SMC N terminal domain
XM_021468356.1 → XP_021324031.1 rho-associated, coiled-coil containing protein kinase 2b isoform X1

UniProtKB/TrEMBL

A0A8M9PP53

Conserved Domains (4) summary

cd00029
Location:1320 → 1374

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd01242
Location:1212 → 1316

PH_ROCK; Rho-associated coiled-coil containing protein kinase pleckstrin homology (PH) domain

cd05596
Location:51 → 402

STKc_ROCK; Catalytic domain of the Serine/Threonine Kinase, Rho-associated coiled-coil containing protein kinase

cl25732
Location:432 → 1097

SMC_N; RecF/RecN/SMC N terminal domain
XM_021468357.1 → XP_021324032.1 rho-associated, coiled-coil containing protein kinase 2b isoform X2

UniProtKB/TrEMBL

A0A8M9PBK6

Conserved Domains (4) summary

cd00029
Location:1320 → 1374

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd01242
Location:1212 → 1316

PH_ROCK; Rho-associated coiled-coil containing protein kinase pleckstrin homology (PH) domain

cd05596
Location:51 → 402

STKc_ROCK; Catalytic domain of the Serine/Threonine Kinase, Rho-associated coiled-coil containing protein kinase

cl25732
Location:432 → 1097

SMC_N; RecF/RecN/SMC N terminal domain
XM_017352434.2 → XP_017207923.1 rho-associated, coiled-coil containing protein kinase 2b isoform X5

UniProtKB/TrEMBL

A0A8M6YWK7

Conserved Domains (7) summary

cd11638
Location:494 → 560

HR1_ROCK2; Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Rho-associated coiled-coil containing protein kinase 2

smart00220
Location:78 → 340

S_TKc; Serine/Threonine protein kinases, catalytic domain

COG1196
Location:432 → 1097

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

cd00029
Location:1253 → 1307

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd01242
Location:1145 → 1249

PH_ROCK; Rho-associated coiled-coil containing protein kinase pleckstrin homology (PH) domain

cd05596
Location:51 → 402

STKc_ROCK; Catalytic domain of the Serine/Threonine Kinase, Rho-associated coiled-coil containing protein kinase

pfam08912
Location:967 → 1034

Rho_Binding; Rho Binding
XM_017352433.2 → XP_017207922.1 rho-associated, coiled-coil containing protein kinase 2b isoform X4

UniProtKB/TrEMBL

A0A8M6YUR0

Conserved Domains (7) summary

cd11638
Location:494 → 560

HR1_ROCK2; Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Rho-associated coiled-coil containing protein kinase 2

smart00220
Location:78 → 340

S_TKc; Serine/Threonine protein kinases, catalytic domain

COG1196
Location:432 → 1097

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

cd00029
Location:1320 → 1374

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd01242
Location:1212 → 1316

PH_ROCK; Rho-associated coiled-coil containing protein kinase pleckstrin homology (PH) domain

cd05596
Location:51 → 402

STKc_ROCK; Catalytic domain of the Serine/Threonine Kinase, Rho-associated coiled-coil containing protein kinase

pfam08912
Location:967 → 1034

Rho_Binding; Rho Binding
XM_009294697.3 → XP_009292972.1 rho-associated, coiled-coil containing protein kinase 2b isoform X10

UniProtKB/TrEMBL

A0A8M3AT74

Conserved Domains (5) summary

COG1340
Location:163 → 435

COG1340; Uncharacterized coiled-coil protein, contains DUF342 domain [Function unknown]

cd00029
Location:768 → 822

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd01242
Location:660 → 764

PH_ROCK; Rho-associated coiled-coil containing protein kinase pleckstrin homology (PH) domain

pfam00169
Location:661 → 753

PH; PH domain

pfam08912
Location:415 → 482

Rho_Binding; Rho Binding
XM_021468358.1 → XP_021324033.1 rho-associated, coiled-coil containing protein kinase 2b isoform X2

UniProtKB/TrEMBL

A0A8M9PBK6

Conserved Domains (4) summary

cd00029
Location:1320 → 1374

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd01242
Location:1212 → 1316

PH_ROCK; Rho-associated coiled-coil containing protein kinase pleckstrin homology (PH) domain

cd05596
Location:51 → 402

STKc_ROCK; Catalytic domain of the Serine/Threonine Kinase, Rho-associated coiled-coil containing protein kinase

cl25732
Location:432 → 1097

SMC_N; RecF/RecN/SMC N terminal domain
XM_009294695.3 → XP_009292970.1 rho-associated, coiled-coil containing protein kinase 2b isoform X7

See identical proteins and their annotated locations for XP_009292970.1

UniProtKB/TrEMBL

A0A8M1NDD8, F1R417

Related

ENSDARP00000136393.1, ENSDART00000157427.2

Conserved Domains (7) summary

smart00220
Location:78 → 340

S_TKc; Serine/Threonine protein kinases, catalytic domain

COG1196
Location:432 → 1097

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

cd00029
Location:1253 → 1307

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd01242
Location:1145 → 1249

PH_ROCK; Rho-associated coiled-coil containing protein kinase pleckstrin homology (PH) domain

cd05596
Location:51 → 402

STKc_ROCK; Catalytic domain of the Serine/Threonine Kinase, Rho-associated coiled-coil containing protein kinase

pfam08912
Location:967 → 1034

Rho_Binding; Rho Binding

cl00087
Location:494 → 560

HR1; Protein kinase C-related kinase homology region 1 (HR1) domain that binds Rho family small GTPases
XM_017352437.2 → XP_017207926.1 rho-associated, coiled-coil containing protein kinase 2b isoform X9

UniProtKB/TrEMBL

A0A8M6Z372

Conserved Domains (5) summary

smart00220
Location:78 → 340

S_TKc; Serine/Threonine protein kinases, catalytic domain

COG1196
Location:432 → 1080

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

cd05596
Location:51 → 402

STKc_ROCK; Catalytic domain of the Serine/Threonine Kinase, Rho-associated coiled-coil containing protein kinase

pfam08912
Location:967 → 1034

Rho_Binding; Rho Binding

cl00087
Location:494 → 560

HR1; Protein kinase C-related kinase homology region 1 (HR1) domain that binds Rho family small GTPases