smad3a SMAD family member 3a [Danio rerio (zebrafish)] - Gene

Summary

Official Symbol: smad3aprovided by ZNC
Official Full Name: SMAD family member 3aprovided by ZNC
Primary source: ZFIN:ZDB-GENE-000509-3
See related: Ensembl:ENSDARG00000036096 AllianceGenome:ZFIN:ZDB-GENE-000509-3
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Danio rerio
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; Danionidae; Danioninae; Danio
Also known as: madh3; smad3; madh3a; wu:fa99e03
Summary: Enables sequence-specific DNA binding activity. Acts upstream of or within regulation of DNA-templated transcription. Predicted to be located in cytoplasm and nucleus. Predicted to be part of heteromeric SMAD protein complex. Is expressed in immature eye; integument; somite; and tail bud. Human ortholog(s) of this gene implicated in Loeys-Dietz syndrome 3; Lynch syndrome; and pancreatic cancer. Orthologous to human SMAD3 (SMAD family member 3). [provided by Alliance of Genome Resources, Nov 2024]
Orthologs: all
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Genomic context

Location:: chromosome: 7

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCz11 (GCF_000002035.6)	7	NC_007118.7 (34106111..34149234, complement)
105	previous assembly	GRCz10 (GCF_000002035.5)	7	NC_007118.6 (33834961..33878084, complement)

Chromosome 7 - NC_007118.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
Description: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
BioProject: PRJEB1986
Analysis date: Fri Dec 8 19:48:10 2017

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Data suggest that Clock1a coordinates mesoderm development and primitive hematopoiesis in embryos by up-regulating Nodal-Smad3 signaling; Clock1a alterations produce embryonic defects with shortened body length, lack of ventral tail fin, or partial defect of the eyes; Clock1a activates Smad3a promoter via its E-box1 element. (Clock1a = clock circadian regulator a; Nodal = nodal modulator 1; Smad3a = SMAD family member 3a)
Title: Clock1a affects mesoderm development and primitive hematopoiesis by regulating Nodal-Smad3 signaling in the zebrafish embryo.
Smad3 is mainly active in post-mitotic, non-proliferating cells with a role in TGF-beta control of zebrafish spinal cord development
Title: A Smad3 transgenic reporter reveals TGF-beta control of zebrafish spinal cord development.
Smad2/3 activities play important roles not only in mesendodermal development but also in neural development during early vertebrate embryogenesis
Title: Smad2/3 activities are required for induction and patterning of the neuroectoderm in zebrafish.
Nodal signaling and mesendoderm induction depend on Smad2/3 and suggest that transforming growth factor-beta signals other than Nodal also contribute to Smad2/3 signaling and embryonic patterning.
Title: smad2 and smad3 are required for mesendoderm induction by transforming growth factor-beta/nodal signals in zebrafish.
although the role of Smad proteins in mediating Nodal signaling is well-documented, the functional characterization of Ttrap provides insight into a novel Smad partner that plays an essential role in the fine-tuning of this signal transduction cascade
Title: Ttrap is an essential modulator of Smad3-dependent Nodal signaling during zebrafish gastrulation and left-right axis determination.

Submit: New GeneRIF Correction

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

fk72e02.x1 (e-PCR)
- UniSTS:201475

G47783 (e-PCR)
- UniSTS:108552

fa99e03 (e-PCR)
- UniSTS:188955

Gene Ontology Provided by ZFIN

Function	Evidence Code	Pubs
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables I-SMAD binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables sequence-specific DNA binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in SMAD protein signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in activin receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in anatomical structure morphogenesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in cell differentiation	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of mesoderm development	IGI Inferred from Genetic Interaction more info
acts_upstream_of_or_within mesoderm development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of mesoderm development	IMP Inferred from Mutant Phenotype more info
acts_upstream_of mesoderm development	TAS Traceable Author Statement more info
involved_in nodal signaling pathway	TAS Traceable Author Statement more info
involved_in positive regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of primitive hemopoiesis	IGI Inferred from Genetic Interaction more info
acts_upstream_of_or_within primitive hemopoiesis	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of primitive hemopoiesis	IMP Inferred from Mutant Phenotype more info
acts_upstream_of primitive hemopoiesis	TAS Traceable Author Statement more info
acts_upstream_of_or_within regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of DNA-templated transcription	IPI Inferred from Physical Interaction more info	PubMed
involved_in transforming growth factor beta receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
part_of heteromeric SMAD protein complex	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of transcription regulator complex	IEA Inferred from Electronic Annotation more info
part_of transcription regulator complex	TAS Traceable Author Statement more info	PubMed

General protein information

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Preferred Names: mothers against decapentaplegic homolog 3a

Names: MAD homolog 3; MAD homolog 3a

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_131571.2 → NP_571646.1 mothers against decapentaplegic homolog 3a

See identical proteins and their annotated locations for NP_571646.1

Status: PROVISIONAL

Source sequence(s)

BC129150

UniProtKB/TrEMBL

A1L1N7, Q8AY15

Related

ENSDARP00000045373.5, ENSDART00000045374.8

Conserved Domains (2) summary

cd10491
Location:7 → 131

MH1_SMAD_2_3; N-terminal Mad Homology 1 (MH1) domain in SMAD2 and SMAD3

cd10985
Location:224 → 414

MH2_SMAD_2_3; C-terminal Mad Homology 2 (MH2) domain in SMAD2 and SMAD3