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Platform GPL10111 Query DataSets for GPL10111
Status Public on Oct 07, 2010
Title Agilent-025188 Custom Agilent Myzus Persicae Array
Technology type in situ oligonucleotide
Distribution custom-commercial
Organism Myzus persicae
Manufacturer Agilent
Manufacture protocol The custom microarray used in this study was designed using the Agilent eArray platform (Agilent Technologies) by the Georg Jander Lab and is based on a previously described array containing probes for >10, 000 M. persicae unigenes produced by Sanger sequencing (Ramsey, Wilson et al. 2007) augmented with an additional 30,517 probe set designed on EST unigene sequences identified in a 454 sequencing project (Ramsey, Rider et al. 2010). The final slide layout consists of four arrays of 45,220 60-mer probes and these are produced by Agilent by in situ oligonucleotide synthesis. References: Ramsey, J. S., D. S. Rider, et al. (2010). "Comparative analysis of detoxification enzymes in Acyrthosiphon pisum and Myzus persicae." Insect Molecular Biology 19: 155-164. Ramsey, J. S., A. C. C. Wilson, et al. (2007). "Genomic resources for Myzus persicae: EST sequencing, SNP identification, and microarray design." BMC Genomics 8.
 
Description All sequences on which the array probes are designed are publically available at http://www.aphidbase.com/aphidbase/downloads under Myzus454(fasta) and MyzusSanger(fasta).
 
Submission date Feb 26, 2010
Last update date Oct 07, 2010
Contact name Chris Bass
E-mail(s) chris.bass@rothamsted.ac.uk
Phone 01582763133
Organization name Rothamsted Research
Department Biological Chemistry
Street address West Common
City Harpenden
State/province Hertfordshire
ZIP/Postal code AL52JQ
Country United Kingdom
 
Samples (16) GSM516213, GSM516214, GSM516215, GSM516216, GSM516217, GSM516218 
Series (2)
GSE20542 Comparison of two clones of Myzus Persicae (Sulzer) (Hemiptera: Aphididae)
GSE24629 Comparison of two clones (4106a and FRC) of Myzus Persicae (Sulzer) (Hemiptera: Aphididae)

Data table header descriptions
ID
accessions
SEQUENCE
ControlType
ProbeName
GeneName
SystematicName
Description
SPOT_ID

Data table
ID accessions SEQUENCE ControlType ProbeName GeneName SystematicName Description SPOT_ID
1 1 GE_BrightCorner GE_BrightCorner GE_BrightCorner -- control
2 1 DarkCorner DarkCorner DarkCorner -- control
3 1 DarkCorner DarkCorner DarkCorner -- control
4 1 DarkCorner DarkCorner DarkCorner -- control
5 1 DarkCorner DarkCorner DarkCorner -- control
6 1 DarkCorner DarkCorner DarkCorner -- control
7 1 DarkCorner DarkCorner DarkCorner -- control
8 1 DarkCorner DarkCorner DarkCorner -- control
9 1 DarkCorner DarkCorner DarkCorner -- control
10 1 DarkCorner DarkCorner DarkCorner -- control
11 1 DarkCorner DarkCorner DarkCorner -- control
12 AGTTGTTCTTCACTTATGATGGATAAAACTTTTTTGATTTATCCTTAGCGTCCCGGCCGT 0 CUST_27180_PI410703081 454Myzus_73925 454Myzus_73925 Unknown 454Myzus_73925
13 CAAACATCATTTGAATTATGCGTGTGGCATTAACCCACAATTTTTAATGTATAGTTCGCC 0 CUST_19711_PI410703081 ACYPI50170 454Myzus_62489 Unknown 454Myzus_62489
14 jander|contig5175 TTGGGTTTTGGTCCACTTGAACCATGATGACGCTTTTGATACATCTGCAAATTGTAAAAT 0 M_persicae5175a unknown protein contig5175 unknown protein contig5175
15 jander|contig8039 GTGCATGCTGAAATGTTAACCATGAGATTTGTACAATTTGACACGACTTTTGATCATCCA 0 M_persicae8039a similar to CG31232-PA, isoform A contig8039 similar to CG31232-PA, isoform A contig8039
16 GTTTAGAACCCAAATATAGGCTTCAAAGGCACGGACTTTTCGAAAAGAAACTCAAACCCG 0 CUST_10426_PI410703081 ACYPI009454 454Myzus_33040 LOC100168780 similar to ribosomal protein S24e [Acyrthosiphon pisum] 454Myzus_33040
17 TTTTTTGGTTTTATTTTATACAATCGGATTCTTATATGGTGAAAAAATTAAATGTCTAAC 0 CUST_14299_PI410703081 454Myzus_55377 454Myzus_55377 Unknown 454Myzus_55377
18 jander|contig5224 CCTCTAGCTCTTATGGAAGACCTAGAAGTAGAAACAGCTGCATAATGTGATAACTAATGG 0 M_persicae5224a lethal (2) giant larvae contig5224 lethal (2) giant larvae contig5224
19 TACGAACAGTAAGAATTTAAGTTTATATGTAAGACTGTCAGTGCAATTTCTGTAGTTTGG 0 CUST_21328_PI410703081 454Myzus_64661 454Myzus_64661 Unknown 454Myzus_64661
20 AAATGGCTACAAAGTTGATAATGTTTGCCGCTTTGTTCGGGCACATGGCCTTTGCTTACC 0 CUST_9567_PI410703081 ACYPI000372 454Myzus_32113 LOC100158955 similar to cuticle protein [Acyrthosiphon pisum] 454Myzus_32113

Total number of rows: 45220

Table truncated, full table size 7020 Kbytes.




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