|
| Status |
Public on Jul 29, 2013 |
| Title |
Array 1_4: 4106a_4 cy3 + FRC_4 cy5 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
ten whole aphids 15 days old
|
| Organism |
Myzus persicae |
| Characteristics |
clone: FRC
tissue: whole organism
age: 15 d
|
| Treatment protocol |
Clones reared as above (both clones were untreated)
|
| Growth protocol |
Both M. persicae clones used in this study were originally established from a single parthenogenetic female collected from a field populations and reared on Chinese cabbage leaves (Brassica napus L var chinensis cv Tip-Top) in small plastic box-cages maintained at 18°C under a 16:8 h light:dark regime. Aphids of similar age were regularly transferred to new boxes in order to rear them as distinct cohorts.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions. Genomic DNA was removed by DNase I digestion using the DNA-free DNase Treatment and Removal Reagent (Ambion).
|
| Label |
cy5
|
| Label protocol |
Two micrograms of each RNA sample was used to generate labeled cRNA, this was prepared, hybridized onto arrays and these were washed and scanned exactly as described in the Agilent Quick Amp Labeling Protocol (Version 5.7) which can be downloaded from http://www.chem.agilent.com/en-US/products/instruments/dnamicroarrays/pages/gp11617.aspx.
|
| |
|
| Channel 2 |
| Source name |
ten whole aphids 15 days old
|
| Organism |
Myzus persicae |
| Characteristics |
clone: 4106a
tissue: whole organism
age: 15 d
|
| Treatment protocol |
Clones reared as above (both clones were untreated)
|
| Growth protocol |
Both M. persicae clones used in this study were originally established from a single parthenogenetic female collected from a field populations and reared on Chinese cabbage leaves (Brassica napus L var chinensis cv Tip-Top) in small plastic box-cages maintained at 18°C under a 16:8 h light:dark regime. Aphids of similar age were regularly transferred to new boxes in order to rear them as distinct cohorts.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions. Genomic DNA was removed by DNase I digestion using the DNA-free DNase Treatment and Removal Reagent (Ambion).
|
| Label |
cy3
|
| Label protocol |
Two micrograms of each RNA sample was used to generate labeled cRNA, this was prepared, hybridized onto arrays and these were washed and scanned exactly as described in the Agilent Quick Amp Labeling Protocol (Version 5.7) which can be downloaded from http://www.chem.agilent.com/en-US/products/instruments/dnamicroarrays/pages/gp11617.aspx.
|
| |
|
| |
| Hybridization protocol |
Two micrograms of each RNA sample was used to generate labeled cRNA, this was prepared, hybridized onto arrays and these were washed and scanned exactly as described in the Agilent Quick Amp Labeling Protocol (Version 5.7) which can be downloaded from http://www.chem.agilent.com/en-US/products/instruments/dnamicroarrays/pages/gp11617.aspx.
|
| Scan protocol |
Scanned on an Agilent G2505C scanner
Images were quantified using Agilent Feature Extraction Software (version 10.5.1.1)
|
| Data processing |
Agilent Feature Extraction Software (v 10.5.1.1) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Oct 12, 2010 |
| Last update date |
Jul 29, 2013 |
| Contact name |
Chris Bass |
| E-mail(s) |
chris.bass@rothamsted.ac.uk
|
| Phone |
01582763133
|
| Organization name |
Rothamsted Research
|
| Department |
Biological Chemistry
|
| Street address |
West Common
|
| City |
Harpenden |
| State/province |
Hertfordshire |
| ZIP/Postal code |
AL52JQ |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL10111 |
| Series (1) |
| GSE24629 |
Comparison of two clones (4106a and FRC) of Myzus Persicae (Sulzer) (Hemiptera: Aphididae) |
|
