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Status
Public on Aug 19, 2012
Title
Kanazawa Univ. Human Liver chip 10k
Technology type
spotted DNA/cDNA
Distribution
non-commercial
Organism
Homo sapiens Manufacturer
First Department of Internal Medmme, Kanazawa University
Manufacture protocol
see manufacturer's website
Description
In addition to in-house cDNA microarray slides consisting 1080 cDNA clones as previously described（Gastroenterology. 2001 Mar;120(4):955-66, we made a new cDNA microarray slide for detailed analysis of the signaling pathway of metabolism and enzyme function in liver disease. Besides cDNA microarray analysis, a total of 256 550 tags were obtained from hepatic SAGE libraries (derived from normal liver, CH-C, CH-C related HCC, CH-B, CH-B related HCC respectively), including 52 149 unique tags. Among these, 16 916 tags expressing more than two hits were selected to avoid the effect of sequencing errors in the libraries. From these candidate genes, 9,614 non-redundant clones were obtained from Incyte Genomics (Incyte Corporation, Beverly, MA, USA), Clontech (Nippon Becton Dickinson, Tokyo, Japan), and Invitrogen (Invitrogen Japan K.K., Tokyo, Japan). Each clone was sequence validated and PCR amplified by Dragon Genomics (Takara Bio, Otsu, Japan), and the cDNA microarray slides (Liver chip 10k) were constructed using SPBIO 2000 (Hitachi Software, Fukuoka, Japan) as described previously.
Submission date
May 15, 2011
Last update date
Aug 19, 2012
Contact name
Masao Honda
E-mail(s)
mhondag@gmail.com Phone
0762652243
Organization name
Kanazawa University
Department
Gastroenterology
Street address
Takara-Machi 13-1
City
Kanazawa
ZIP/Postal code
920-8641
Country
Japan
Samples (153)
GSM724248 , GSM724249 , GSM724250 , GSM724251 , GSM724252 , GSM724253
GSM724254 ,
GSM724255 ,
GSM724256 ,
GSM724257 ,
GSM724258 ,
GSM724259 ,
GSM724260 ,
GSM724261 ,
GSM724262 ,
GSM724263 ,
GSM724264 ,
GSM724265 ,
GSM724266 ,
GSM724267 ,
GSM724268 ,
GSM724269 ,
GSM724270 ,
GSM724271 ,
GSM724272 ,
GSM724273 ,
GSM724274 ,
GSM724275 ,
GSM724276 ,
GSM724277 ,
GSM724278 ,
GSM724279 ,
GSM724280 ,
GSM724281 ,
GSM724282 ,
GSM724283 ,
GSM724284 ,
GSM724285 ,
GSM724286 ,
GSM724287 ,
GSM724288 ,
GSM724289 ,
GSM724290 ,
GSM724291 ,
GSM724292 ,
GSM724293 ,
GSM724294 ,
GSM724295 ,
GSM1077488 ,
GSM1077489 ,
GSM1077490 ,
GSM1077491 ,
GSM1077492 ,
GSM1077493 ,
GSM1077494 ,
GSM1077495 ,
GSM1077496 ,
GSM1077497 ,
GSM1077498 ,
GSM1077499 ,
GSM1077500 ,
GSM1077501 ,
GSM1077502 ,
GSM1077503 ,
GSM1077504 ,
GSM1077505 ,
GSM1077506 ,
GSM1077507 ,
GSM1077508 ,
GSM1077509 ,
GSM1077510 ,
GSM1077511 ,
GSM1077512 ,
GSM1077513 ,
GSM1077514 ,
GSM1077515 ,
GSM1077516 ,
GSM1077517 ,
GSM1077518 ,
GSM1077519 ,
GSM1077520 ,
GSM1077521 ,
GSM1077522 ,
GSM1077523 ,
GSM1077524 ,
GSM1077525 ,
GSM1077526 ,
GSM1077527 ,
GSM1077528 ,
GSM1077529 ,
GSM1077530 ,
GSM1077531 ,
GSM1077532 ,
GSM1077533 ,
GSM1077534 ,
GSM1077535 ,
GSM1077536 ,
GSM1077537 ,
GSM1077538 ,
GSM1077539 ,
GSM1077540 ,
GSM1077541 ,
GSM1077542 ,
GSM1077543 ,
GSM1077544 ,
GSM1077545 ,
GSM1077546 ,
GSM1077547 ,
GSM1077548 ,
GSM1077549 ,
GSM1077550 ,
GSM1077551 ,
GSM1077552 ,
GSM1077553 ,
GSM1077554 ,
GSM1077555 ,
GSM1077556 ,
GSM1077557 ,
GSM1077558 ,
GSM1077559 ,
GSM1077560 ,
GSM1077561 ,
GSM1077562 ,
GSM1077563 ,
GSM1077564 ,
GSM1077565 ,
GSM1077566 ,
GSM1077567 ,
GSM1077568 ,
GSM1077569 ,
GSM1077570 ,
GSM1077571 ,
GSM1077572 ,
GSM1077573 ,
GSM1077574 ,
GSM1077575 ,
GSM1077576 ,
GSM1077577 ,
GSM1077578 ,
GSM1077579 ,
GSM1077580 ,
GSM1077581 ,
GSM1077582 ,
GSM1077583 ,
GSM1077584 ,
GSM1077585 ,
GSM1077586 ,
GSM1077587 ,
GSM1077588 ,
GSM1077589 ,
GSM1077590 ,
GSM1077591 ,
GSM1077592 Series (2)
GSE29302 Acyclic retinoid targets platelet-derived growth factor signaling in the prevention of hepatic fibrosis and hepatocellular carcinoma development
GSE44074 Gene expression profiling of hepatitis B- and hepatitis C-related hepatocellular carcinoma using graphical Gaussian modeling
Data table header descriptions ID Stock No. Stock No.
Row Row ID
Column Column ID
Location No. Location ID
Gene Name Gene name
Symbol GB_ACC GenBank Accession number
SPOT_ID
Data table ID Stock No. Row Column Location No. Gene Name Symbol GB_ACC SPOT_ID 1001
1
1
15
41501
Ribosomal protein L31
RPL31
NM_000993 1002
2
1
15
121501
-- non annotated or unknown genes
1003
3
1
15
201501
RNA binding motif protein 4
RBM4
NM_002896 1004
4
1
15
281501
Interleukin 15 receptor, alpha
IL15RA
NM_002189 1005
5
1
15
361501
-- non annotated or unknown genes
1006
6
1
15
441501
Cyclin B1
CCNB1
NM_031966 1007
7
1
14
41401
Interleukin 15 receptor, alpha
IL15RA
NM_002189 1008
8
1
14
121401
-- non annotated or unknown genes
1009
9
1
14
201401
Cyclin D2
CCND2
NM_001759 1010
10
1
14
281401
E2F transcription factor 4, p107/p130-binding
E2F4
NM_001950 1011
11
1
14
361401
Suppression of tumorigenicity 13 (colon carcinoma) (Hsp70 interacting protein)
ST13
NM_003932 1012
12
1
14
441401
Baculoviral IAP repeat-containing 2
BIRC2
NM_001166 1013
13
1
15
21501
Defender against cell death 1
DAD1
NM_001344 1014
14
1
15
101501
-- non annotated or unknown genes
1015
15
1
15
181501
TRAF family member-associated NFKB activator
TANK
NM_133484 1016
16
1
15
261501
D4, zinc and double PHD fingers family 2
DPF2
NM_006268 1017
17
1
15
341501
Tumor protein p53 binding protein, 2
TP53BP2
NM_005426 1018
18
1
15
421501
Interleukin 8
IL8
NM_000584 1019
19
1
14
21401
Tumor protein p53 (Li-Fraumeni syndrome)
TP53
NM_000546 1020
20
1
14
101401
Bone morphogenetic protein 6
BMP6
NM_001718
9643 758 Kbytes .Supplementary data files not provided
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