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Status
Public on Jul 22, 2015
Title
29K Cotton Genome Array
Technology type
spotted DNA/cDNA
Distribution
custom-commercial
Organism
Gossypium hirsutum
Manufacturer
CapitalBio Corporation,National Engineering Research Center for Beijing Biochip Technology,18 Life Science Parkway, Beijing 102206, China
Manufacture protocol
1.29K Cotton Genome cDNA microarray constructed by Peking University and CapitalBio Corp. The Peking University construct cDNA library and provide PCR products (Shi et al., 2006). And PCR products were printed in SmartArrayTM (CapitalBio Corp., Beijing, China). 2. Hex was used as positive control. 3. 50% DMSO was used as negative control. 4.Several housekeeping genes were used as hybridization positive control. 5.Eight sequence derived from intergenic regions of the yeast genome showing no significant homology with any existing cotton sequences in GenBank were used as external controls.
Submission date
Jul 26, 2011
Last update date
Jul 22, 2015
Contact name
Lu You Yuan
E-mail(s)
youluyuan@hotmail.com
Phone
86-372-2525350
Organization name
Cotton Research Institute, Chinese Academy of Agricultyral Science
Department
Molecular Breeding
Lab
Cotton Lab
Street address
Huanghe Road 38#
City
Anyang
State/province
Henan
ZIP/Postal code
455000
Country
China
Samples (15)
GSM767231 , GSM767232 , GSM767233 , GSM767234 , GSM767235 , GSM767236
Series (1)
GSE30944
cDNA array expression in cotton fiber secondary cell wall development from two germplasm lines that differ in fiber strength
Data table header descriptions
ID
GB_ACC
GenBank accession number of sequence used to design cDNA array probe
Description
Gene description
SPOT_ID
Gene symbols
Data table
ID
GB_ACC
Description
SPOT_ID
CM020A01
NC
--NC
CM034G09
NC
--NC
CM048F4
NC
--NC
CM061D06
NC
--NC
CM115A05
NC
--NC
HEX
spot and immobilization positive control
--spot and immobilization positive control
50%DMSO
hybridization negative control,spot solution
--hybridization negative control,spot solution
Y1
Yeast intergenic sequence 1, spike RNA
--Yeast intergenic sequence 1, spike RNA
Y2
Yeast intergenic sequence 2, spike RNA
--Yeast intergenic sequence 2, spike RNA
Y3
Yeast intergenic sequence 3, spike RNA
--Yeast intergenic sequence 3, spike RNA
Y4
Yeast intergenic sequence 4, spike RNA
--Yeast intergenic sequence 4, spike RNA
Y5
Yeast intergenic sequence 5, spike RNA
--Yeast intergenic sequence 5, spike RNA
Y6
Yeast intergenic sequence 6, spike RNA
--Yeast intergenic sequence 6, spike RNA
Y7
Yeast intergenic sequence 7, spike RNA
--Yeast intergenic sequence 7, spike RNA
Y8
Yeast intergenic sequence 8, spike RNA
--Yeast intergenic sequence 8, spike RNA
384P01_A_01
ES834740
2OG-Fe(II) oxygenase [Medicago truncatula]
384P01_A_02
ES801680
putative nicotiana eukaryotic translation initiation factor 4A [Oryza sativa (japonica cultivar-group)]
384P01_A_03
ES834761
peptidase [Arabidopsis thaliana]
384P01_A_04
EY196057
384P01_A_05
ES834762
unknown [Arabidopsis thaliana]
Total number of rows: 28196 Table truncated, full table size 2524 Kbytes .
Supplementary data files not provided