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| Status |
Public on Jul 22, 2015 |
| Title |
cDNA array expression in cotton fiber secondary cell wall development from two germplasm lines that differ in fiber strength |
| Organism |
Gossypium hirsutum |
| Experiment type |
Expression profiling by array
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| Summary |
Comparison of gene expression profiles in cotton fiber development (from 10 to 23 DPA) of two lines. Line 1 was excellent fiber strength, denoted with H; Line 2 was poor fiber strength, denoted withL.
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| Overall design |
Analysis used H20 RNA as control samples for comparison to the experimental samples taken at 5, 10, 15 and 23 DPA. Indirect comparisons were made across multiple arrays with raw data pulled from different channels for data analysis and comparison to the control data.Cy3 and Cy5 dyes were used as follows:Set 1:H10-Cy5 vs H20-Cy3, Set 1(dye swap):H10-Cy3 vs H20-Cy5; Set 2:H15-Cy5 vs H20-Cy3,Set 2 (dya swap):H15-Cy3 vs H20-Cy5; Set 3:H23-Cy5 vs H20-Cy3, Set 3 (dya swap):H23-Cy3 vs H20-Cy5; Set 4:L10-Cy5 vs H20-Cy3, Set 4 (dya swap):L10-Cy3 vs H20-Cy5; Set 5:L15-Cy5 vs H20-Cy3, Set 5 (dya swap):L15-Cy3 vs H20-Cy5; Set 6:L20-Cy5 vs H20-Cy3, Set 6 (dya swap):L20-Cy3 vs H20-Cy5; Set 7:L23-Cy5 vs H20-Cy3, Set 7 (dya swap):L23-Cy3 vs H20-Cy5; Set 8:H20-Cy5 vs H20-Cy3.
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| Contributor(s) |
Ji Z, Shang H, Yuan Y |
| Citation missing |
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| Submission date |
Jul 26, 2011 |
| Last update date |
Jul 22, 2015 |
| Contact name |
Lu You Yuan |
| E-mail(s) |
youluyuan@hotmail.com
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| Phone |
86-372-2525350
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| Organization name |
Cotton Research Institute, Chinese Academy of Agricultyral Science
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| Department |
Molecular Breeding
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| Lab |
Cotton Lab
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| Street address |
Huanghe Road 38#
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| City |
Anyang |
| State/province |
Henan |
| ZIP/Postal code |
455000 |
| Country |
China |
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| Platforms (1) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA146249 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE30944_RAW.tar |
35.1 Mb |
(http)(custom) |
TAR (of LSR, TXT) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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