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Platform GPL14115 Query DataSets for GPL14115
Status Public on Sep 01, 2011
Title CAASICS-plant microRNA -miRBase 9.1
Technology type spotted oligonucleotide
Distribution custom-commercial
Organisms Physcomitrium patens; Populus trichocarpa; Arabidopsis thaliana; Glycine max; Medicago truncatula; Oryza sativa; Saccharum officinarum; Sorghum bicolor; Zea mays
Manufacturer Beijing Capitalbio
Manufacture protocol The plant miRNA microarray V2.0 is based on 994 sequences in miRBase 9.1 (Feberary 2007) of Sanger Database. 348 unique miRNAs were retained after removing the redundant sequences. Coupled with 4 miRNAs predicted by Zhu Jian-Kang on Cell Journal, and 74 miRNAs predicted by Chen Fan (CAS), there are 426 miRNAs probes in all on the array. These probes were spotted on a piece of 75mm multiply 25mm, chemical modified glass using SmartArrayTM (CapitalBio Corp., Beijing, China ) machine. Eight manufactered RNA with 30nt in length were used as exogenous controls (Zip5, Zip13, Zip15, Zip21, Zip23, Zip25, Y2, Y3). Hex was used as positive control and 50% DMSO was negetive control.
The whole array was divided as 4 subarray. There are 18 rows and 21 columns in each subarray. The diameter of spots was 150 micrometer with 225 micrometer intervals between two spots. Each probe was spotted in three technical replicants.
Support glass
Coating unknown
 
 
Submission date Aug 04, 2011
Last update date Sep 01, 2011
Contact name Chuanxiao Xie
E-mail(s) cxxie@caas.net.cn
Organization name Chinese Academy of Agricultural Sciences
Department Institute of Crop Science
Lab Center for maize study
Street address Zhong-guan-cun South St. No12
City Beijing
ZIP/Postal code 100081
Country China
 
Samples (12) GSM774082, GSM777801, GSM777802, GSM777803, GSM777804, GSM777805 
Series (2)
GSE31373 miRNA expression data from low nitrate culture in leaf and root of maize
GSE31492 miRNA expression data from low nitrate cultures in maize

Data table header descriptions
ID
miRNA_ID Source miRNAs of each spot on the array (blank indicate different controls)
SEQUENCE Probe sequence of each spot on the array
SPOT_ID

Data table
ID miRNA_ID SEQUENCE SPOT_ID
1 GACCACCTTGCGATCGGGTACAGC UNKNOWN
2 GACCACCTTGCGATCGGGTACAGC UNKNOWN
3 GACCACCTTGCGATCGGGTACAGC UNKNOWN
4 CAGCGGTAGACCACCTATCGTGCG UNKNOWN
5 CAGCGGTAGACCACCTATCGTGCG UNKNOWN
6 CAGCGGTAGACCACCTATCGTGCG UNKNOWN
7 GACCGGTATGCGACCTGGTATGCG UNKNOWN
8 GACCGGTATGCGACCTGGTATGCG UNKNOWN
9 GACCGGTATGCGACCTGGTATGCG UNKNOWN
10 TGCGATCGCAGCGGTAACCTGACC UNKNOWN
11 TGCGATCGCAGCGGTAACCTGACC UNKNOWN
12 TGCGATCGCAGCGGTAACCTGACC UNKNOWN
13 CAGCATCGGACCGGTAATCGGACC UNKNOWN
14 CAGCATCGGACCGGTAATCGGACC UNKNOWN
15 CAGCATCGGACCGGTAATCGGACC UNKNOWN
16 GACCATAGTGCGGGTAGGTAGACC UNKNOWN
17 GACCATAGTGCGGGTAGGTAGACC UNKNOWN
18 GACCATAGTGCGGGTAGGTAGACC UNKNOWN
19 AGGTACGAAACGCTAAGAAT UNKNOWN
20 AGGTACGAAACGCTAAGAAT UNKNOWN

Total number of rows: 1503

Table truncated, full table size 56 Kbytes.




Download family Format
SOFT formatted family file(s) SOFTHelp
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Supplementary data files not provided
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