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| Status |
Public on Sep 01, 2011 |
| Title |
miRNA expression data from low nitrate culture in leaf and root of maize |
| Platform organisms |
Physcomitrium patens; Populus trichocarpa; Arabidopsis thaliana; Glycine max; Medicago truncatula; Oryza sativa; Saccharum officinarum; Sorghum bicolor; Zea mays |
| Sample organism |
Zea mays |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Nitrate is the major source of nitrogen available for many crop plants and is often the limiting factor for plant growth and agricultural productivity especially for maize. Many studies have been done identifying the transcriptome changes under low nitrate conditions. However, the microRNAs (miRNAs) varied under nitrate limiting conditions in maize has not been reported. MiRNAs play important roles in abiotic stress responses and nutrient deprivation.
We used the microarray systems to detect miRNAs responding to the chronic nitrate limiting conditions in maize leaves and roots.
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| Overall design |
MicroRNAs (miRNAs) are small, endogenous RNAs that are regulators of gene expression in plants and animals. And, miRNAs have been known for years to be important for phosphate, sulphate and copper deprivation responses in plants. In Arabidopsis, small RNA deep sequencing associated with nitrate response had been analyzed. However, information about the way by which miRNA are regulated by abiotic stresses in general and by low nitrate in particular is unavailable for maize. In this study, we used SmartArrayTM microarray system to detect miRNAs responding to chronic nitrate limiting conditions in roots and leaves in maize. Leaf and root under normal and low nitrate conditions were analyzed with three biological replicates. So there are 12 samples in this study.
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| Contributor(s) |
Xie C, Bi Y, Zhang S, Rothstein SJ, Xu Z, Zhong S, Li X, Li W |
| Citation(s) |
22132192 |
| |
| Submission date |
Aug 15, 2011 |
| Last update date |
Sep 19, 2012 |
| Contact name |
Chuanxiao Xie |
| E-mail(s) |
cxxie@caas.net.cn
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| Organization name |
Chinese Academy of Agricultural Sciences
|
| Department |
Institute of Crop Science
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| Lab |
Center for maize study
|
| Street address |
Zhong-guan-cun South St. No12
|
| City |
Beijing |
| ZIP/Postal code |
100081 |
| Country |
China |
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| Platforms (1) |
| GPL14115 |
CAASICS-plant microRNA -miRBase 9.1 |
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| Samples (12)
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| GSM774082 |
leaf high N and low N biological replicate 1 |
| GSM777801 |
leaf high N and low N biological replicate 2 |
| GSM777802 |
leaf high N and low N biological replicate 3 |
| GSM777803 |
root high N and low N biological replicate 1 |
| GSM777804 |
root high N and low N biological replicate 2 |
| GSM777805 |
root high N and low N biological replicate 3 |
| GSM777806 |
channel exchange leaf high N and low N biological replicate 1 |
| GSM777807 |
channel exchange leaf high N and low N biological replicate 2 |
| GSM777808 |
channel exchange leaf high N and low N biological replicate 3 |
| GSM777809 |
channel exchange root high N and low N biological replicate 1 |
| GSM777810 |
channel exchange root high N and low N biological replicate 2 |
| GSM777811 |
channel exchange root high N and low N biological replicate 3 |
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| This SubSeries is part of SuperSeries: |
| GSE31492 |
miRNA expression data from low nitrate cultures in maize |
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| Relations |
| BioProject |
PRJNA154147 |
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