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Status
Public on Oct 10, 2006
Title
Mussel MytArray 1.0
Technology type
spotted DNA/cDNA
Distribution
non-commercial
Organism
Mytilus galloprovincialis Manufacturer
C.R.I.B.I.-Biotechnology Centre and Dept. Biology (University of Padova)
Support
glass
Coating
aminosilane
Description
MytArray 1.0 is a cDNA platform, that consists of 1,712 different clones obtained from systematic sequencing of multiple Mytilus galloprovincialis tissues cDNA library and 46 unrelated controls (ESTs from Coffea and bacterial species) printed in duplicate and twice per slide. This library was produced using a strategy that allows the selection of a short 3’-end region of mRNAs and the tagging of each transcript with an unique probe. Moreover, since the 3’-end non coding region is the least conserved part of the genes, a microarray constructed in this way greatly reduces the cross hybridization of different mRNAs derived from genes with high homology in the coding region to the same probe. The strategy was also designed to obtain 3’-tags with very uniform size (400-800 bp). This feature ensures that all the cDNA clones of the collection can be PCR-amplified and spotted with comparable efficiency and that production of microarrays with uniform hybridization characteristics be obtained.
Contributor(s)
Venier P , De Pittà C , Marsano F , Pallavicini A , Varotto L , Romualdi C , Viarengo A , Lanfranchi G Citation(s)
17010391 Submission date
Jan 19, 2005
Last update date
Oct 10, 2006
Contact name
Gerolamo Lanfranchi
E-mail(s)
stefano.cagnin@unipd.it Phone
+39-0498276219
Organization name
University of Padova
Department
CRIBI - Biotechnology Center and Biology Department
Lab
Functional Genomics Lab
Street address
Via U. Bassi, 58/B
City
Padova
ZIP/Postal code
35131
Country
Italy
Samples (68)
GSM39225 , GSM39226 , GSM39227 , GSM39228 , GSM39229 , GSM39230
GSM39231 ,
GSM39232 ,
GSM39421 ,
GSM39422 ,
GSM39423 ,
GSM39424 ,
GSM39571 ,
GSM39572 ,
GSM39573 ,
GSM39574 ,
GSM39575 ,
GSM39576 ,
GSM371994 ,
GSM371995 ,
GSM371996 ,
GSM371997 ,
GSM371998 ,
GSM371999 ,
GSM372000 ,
GSM372001 ,
GSM372002 ,
GSM372003 ,
GSM372004 ,
GSM372005 ,
GSM372006 ,
GSM372007 ,
GSM372008 ,
GSM372009 ,
GSM372010 ,
GSM372011 ,
GSM372012 ,
GSM372013 ,
GSM372014 ,
GSM372015 ,
GSM372016 ,
GSM372017 ,
GSM530797 ,
GSM530798 ,
GSM530799 ,
GSM530800 ,
GSM530801 ,
GSM530802 ,
GSM530803 ,
GSM530804 ,
GSM1010992 ,
GSM1010993 ,
GSM1010994 ,
GSM1010995 ,
GSM1010996 ,
GSM1010997 ,
GSM1010998 ,
GSM1010999 ,
GSM1011000 ,
GSM1011001 ,
GSM1011002 ,
GSM1011003 ,
GSM1011004 ,
GSM1011005 ,
GSM1011006 ,
GSM1011007 ,
GSM1011008 ,
GSM1011009 Series (6)
GSE2176 Tissue specificity of gene expression profiling from mussels in normal conditions.
GSE2183 Gene expression profiling of mussels treated with two chemical mixtures
GSE2184 Gene expression profiling of native mussels from Venice lagoon area
GSE14885 Time course study of Mediterranean mussel (M. galloprovincialis, Lmk, 1819) digestive gland treated with okadaic acid
GSE21229 Mussel (Mytilus galloprovincialis) digestive gland tissue: Control vs. Nickel, Chlorpyrifos and their mixture.
GSE41218 How nanomolar doses of combined metal salts (Cd, Cu, Hg) induce DNA damage and transcriptional changes in the gills of Mytilus galloprovincialis.
Data table header descriptions ID
CLONEID cDNA identifier at CRIBI
GB_ACC GenBank Accession number
SPOT_ID
Description cDNA probe description
Cluster_ID Cluster identifier at CRIBI database
Array Column array column position in MytArray 1.0
Array Row array row position in MytArray 1.0
Column column probe position in array
Row row probe position in array
Data table ID CLONEID GB_ACC SPOT_ID Description Cluster_ID Array Column Array Row Column Row 1
Myt01-001A01
AJ516347 Without similarity
GPPD00286
4
1
16
1
2
Myt01-001A01
AJ516347 Without similarity
GPPD00286
4
1
16
9
3
Myt01-001A02
AJ516349 Without similarity
GPPD00288
4
3
16
1
4
Myt01-001A02
AJ516349 Without similarity
GPPD00288
4
3
16
9
5
Myt01-001A03
AJ516350 gi|23491586|dbj|BAC16745.1| calponin [Branchiostoma belcheri]
GPPD00289
4
1
16
2
6
Myt01-001A03
AJ516350 gi|23491586|dbj|BAC16745.1| calponin [Branchiostoma belcheri]
GPPD00289
4
1
16
10
7
Myt01-001A04
AJ516353 gi|27464930|gb|AAO16245.1| hypertension related protein 1-like protein [Mus musculus]
GPPD00292
4
3
16
2
8
Myt01-001A04
AJ516353 gi|27464930|gb|AAO16245.1| hypertension related protein 1-like protein [Mus musculus]
GPPD00292
4
3
16
10
9
Myt01-001A05
AJ516359 Without similarity
GPPD00299
4
1
16
3
10
Myt01-001A05
AJ516359 Without similarity
GPPD00299
4
1
16
11
11
Myt01-001A06
AJ516360 Without similarity
GPPD00300
4
3
16
3
12
Myt01-001A06
AJ516360 Without similarity
GPPD00300
4
3
16
11
13
Myt01-001A07
AJ516361 gi|22758894|gb|AAN05606.1| ribosomal protein L9 [Argopecten irradians]
GPPD00301
4
1
16
4
14
Myt01-001A07
AJ516361 gi|22758894|gb|AAN05606.1| ribosomal protein L9 [Argopecten irradians]
GPPD00301
4
1
16
12
15
Myt01-001A08
AJ516362 gi|19338058|emb|AJ271365.2|MED271365 Mytilus edulis gene for endo-1,4-mannanase, exons 1-6
GPPD00302
4
3
16
4
16
Myt01-001A08
AJ516362 gi|19338058|emb|AJ271365.2|MED271365 Mytilus edulis gene for endo-1,4-mannanase, exons 1-6
GPPD00302
4
3
16
12
17
Myt01-001A09
AJ516363 Without similarity
GPPD00303
4
1
16
5
18
Myt01-001A09
AJ516363 Without similarity
GPPD00303
4
1
16
13
19
Myt01-001A10
AJ516364 gi|9055210|ref|NP_061266.1| eukaryotic translation elongation factor 1 beta [Mus musculus]
GPPD00304
4
3
16
5
20
Myt01-001A10
AJ516364 gi|9055210|ref|NP_061266.1| eukaryotic translation elongation factor 1 beta [Mus musculus]
GPPD00304
4
3
16
13
3840 360 Kbytes .Supplementary data files not provided
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