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| Status |
Public on Oct 10, 2006 |
| Title |
Tissue specificity of gene expression profiling from mussels in normal conditions. |
| Organism |
Mytilus galloprovincialis |
| Experiment type |
Expression profiling by array
|
| Summary |
We examined tissue specificity of gene expression in control mussels. Total RNA was isolated from gills (G), digestive gland (DG), tissues involved in contraction/motility (FML: foot/adductor muscles/ligaments) and reproduction (GM: gonads and mantle) producing four tissue-specific samples. Total RNA was reverse-transcribed and labeled using a Cy3-dCTP and Cy5-dCTP direct incorporation.
Each sample was hybridized in competition with the total pool (equal RNA amounts from G, DG, GM and FML) and the relative abundance of each gene was measured by directly comparing fluorescent signals for each probe. We reversed labeling of pool tissues (reference) and single tissue sample in each experiment, thus carrying out two separate hybridizations for each tissue.
Keywords = mussels
Keywords = tissue specificity
Keywords = normal conditions
Keywords = gene expression profiling
Keywords: ordered
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| Contributor(s) |
Venier P, De Pittà C, Marsano F, Pallavicini A, Varotto L, Romualdi C, Viarengo A, Lanfranchi G |
| Citation(s) |
17010391 |
| |
| Submission date |
Jan 24, 2005 |
| Last update date |
Mar 16, 2012 |
| Contact name |
Gerolamo Lanfranchi |
| E-mail(s) |
stefano.cagnin@unipd.it
|
| Phone |
+39-0498276219
|
| Organization name |
University of Padova
|
| Department |
CRIBI - Biotechnology Center and Biology Department
|
| Lab |
Functional Genomics Lab
|
| Street address |
Via U. Bassi, 58/B
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| City |
Padova |
| ZIP/Postal code |
35131 |
| Country |
Italy |
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| Platforms (1) |
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| Samples (8)
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| GSM39225 |
Gills gene expression profiling from mussels in normal conditions (A) |
| GSM39226 |
Gills gene expression profiling from mussels in normal conditions (B) |
| GSM39227 |
Digestive gland gene expression profiling from mussels in normal conditions (A) |
| GSM39228 |
Digestive gland gene expression profiling from mussels in normal conditions (B) |
| GSM39229 |
Gonads/mantle gene expression profiling from mussels in normal conditions (A) |
| GSM39230 |
Gonads/mantle gene expression profiling from mussels in normal conditions (B) |
| GSM39231 |
Foot/adductor muscles/ligaments gene expression profiling from mussels in normal conditions (A) |
| GSM39232 |
Foot/adductor muscles/ligaments gene expression profiling from mussels in normal conditions (B) |
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| Relations |
| BioProject |
PRJNA91223 |
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